Label-free molecular binding assays using holographic video microscopy

Fook Chiong Cheong, David G. Grier

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Inline holographic video microscopy's resolution for single particle characterization is fine enough to detect molecular-scale coatings on colloidal surfaces, without requiring staining or fluorescent labeling. We demonstrate this approach to molecular binding assay by detecting the binding of avidin to biotinylated polystyrene spheres.

    Original languageEnglish (US)
    Title of host publicationBio-Optics
    Subtitle of host publicationDesign and Application, BODA 2011
    StatePublished - Dec 1 2011
    EventBio-Optics: Design and Application, BODA 2011 - Monterey, CA, United States
    Duration: Apr 4 2011Apr 6 2011

    Publication series

    NameOptics InfoBase Conference Papers
    ISSN (Electronic)2162-2701

    Other

    OtherBio-Optics: Design and Application, BODA 2011
    CountryUnited States
    CityMonterey, CA
    Period4/4/114/6/11

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    ASJC Scopus subject areas

    • Instrumentation
    • Atomic and Molecular Physics, and Optics

    Cite this

    Cheong, F. C., & Grier, D. G. (2011). Label-free molecular binding assays using holographic video microscopy. In Bio-Optics: Design and Application, BODA 2011 (Optics InfoBase Conference Papers).