Label-free DNA microarray bioassays using a near-field scanning microwave microscope

Kiejin Lee, Arsen Babajanyan, Harutyun Melikyan, Chongchel Kim, Seungwan Kim, Jungho Kim, Jung Ha Lee, Barry Friedman, Rastislav Levicky, Sergey Kalachikov

Research output: Contribution to journalArticle

Abstract

A near-field scanning microwave microscope (NSMM) is used to readout and visualize homemade 10-mer oligonucleotide microarrays and an Agilent 60-mer DNA microarray as a realistic test of NSMM applicability to multiplexed sequence analysis. Sensitive characterization of DNA coverage and high resolution mapping of DNA spots in the microarray were realized by measuring the change of microwave reflection coefficient (S11) at about 4GHz operating frequency. Hybridization between target (free) and capture (immobilized) sequences leads to changes in the microwave reflection coefficient, which were measured by the NSMM. These changes are caused by hybridization-induced modification of the dielectric permittivity profile of the DNA film. The dynamic range based on analysis of the 10-mer microarrays is over 3 orders of magnitude with the detection limit estimated below 0.01strands/μm2. The NSMM method should be readily capable of detecting target coverages down to 98% of probe coverage. We also directly image the patterned DNA microarray by NSMM with a 2μm resolution. The complementary optical image of the DNA microarray visualized by using a relative fluorescent intensity metric agrees well with the NSMM results.

Original languageEnglish (US)
Pages (from-to)326-331
Number of pages6
JournalBiosensors and Bioelectronics
Volume42
Issue number1
DOIs
StatePublished - Apr 5 2013

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Keywords

  • DNA bioassay
  • Microwave microscope
  • Oligonucleotide microarrays

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Lee, K., Babajanyan, A., Melikyan, H., Kim, C., Kim, S., Kim, J., Lee, J. H., Friedman, B., Levicky, R., & Kalachikov, S. (2013). Label-free DNA microarray bioassays using a near-field scanning microwave microscope. Biosensors and Bioelectronics, 42(1), 326-331. https://doi.org/10.1016/j.bios.2012.10.027