Kinetics of synaptic transfer from rods and cones to horizontal cells in the salamander retina

W. B. Thoreson, D. Tranchina, P. Witkovsky

Research output: Contribution to journalArticle

Abstract

We examined synaptic transmission between rods or cones and horizontal cells, using perforated patch recording techniques in salamander retinal slices. Experimental conditions were established under which horizontal cells received nearly pure rod or pure cone input. The response-intensity relation for both photoreceptors and horizontal cells was described by a Michaelis-Menten function with an exponent close to 1. A dynamic model was developed for the transduction from photoreceptor voltage to postsynaptic current. The basic model assumes that: (i) photoreceptor light-evoked voltage controls Ca 2+ entry according to a Boltzmann relation; (ii) the rate of glutamate release depends linearly on the voltage-gated Ca2+ current (ICa) in the synaptic terminal; (iii) glutamate concentration in the synaptic cleft reflects the balance of release and reuptake in which reuptake obeys first order kinetics; (iv) the binding of glutamate to its receptor and channel gating are fast compared with glutamate kinetics in the synaptic cleft. The good fit to the model confirms that these are the key features of synaptic transmission from rods and cones. The model accommodated changes in kinetics induced by the glutamate uptake blocker, dihydrokainate. The match between model and response was not improved by including an estimate of α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor desensitization or by making glutamate uptake voltage dependent.

Original languageEnglish (US)
Pages (from-to)785-798
Number of pages14
JournalNeuroscience
Volume122
Issue number3
DOIs
StatePublished - 2003

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Urodela
Vertebrate Photoreceptor Cells
Retina
Glutamic Acid
Synaptic Transmission
Synaptic Potentials
Photoreceptor Cells
Presynaptic Terminals
Light

Keywords

  • Computer simulation
  • L-type calcium current
  • Photoreceptor
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kinetics of synaptic transfer from rods and cones to horizontal cells in the salamander retina. / Thoreson, W. B.; Tranchina, D.; Witkovsky, P.

In: Neuroscience, Vol. 122, No. 3, 2003, p. 785-798.

Research output: Contribution to journalArticle

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