Kinetics of benzo[a]pyrene induced mutagenesis in a highly sensitive Salmonella/microsome assay

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The kinetics of benzo[a]pyrene induced mutagenesis in Salmonella typhimurium TA98 and TA100 were studied using a liquid-phase assay and were compared to kinetics of metabolism of benzo[a]pyrene in the same system. Mutagenesis was terminated by the addition of pyrene + menadione at selected times. Under conditions of the assay using hepatic microsomes from 3-methylcholanthrene pretreated rats, metabolism of benzo[a]pyrene was over 50% complete in 5 min, and metabolism of initial metabolites became appreciable after this time. The revertant frequency (revertants/108 survivors) and the production of tertiary metabolites increased sharply after 5 min and leveled off at 40 min.

Original languageEnglish (US)
Pages (from-to)221-228
Number of pages8
JournalMutation Research/Genetic Toxicology
Issue number3
StatePublished - Mar 1980


ASJC Scopus subject areas

  • Toxicology
  • Genetics

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