Kinetics of benzo[a]pyrene induced mutagenesis in a highly sensitive Salmonella/microsome assay

Research output: Contribution to journalArticle

Abstract

The kinetics of benzo[a]pyrene induced mutagenesis in Salmonella typhimurium TA98 and TA100 were studied using a liquid-phase assay and were compared to kinetics of metabolism of benzo[a]pyrene in the same system. Mutagenesis was terminated by the addition of pyrene + menadione at selected times. Under conditions of the assay using hepatic microsomes from 3-methylcholanthrene pretreated rats, metabolism of benzo[a]pyrene was over 50% complete in 5 min, and metabolism of initial metabolites became appreciable after this time. The revertant frequency (revertants/108 survivors) and the production of tertiary metabolites increased sharply after 5 min and leveled off at 40 min.

Original languageEnglish (US)
Pages (from-to)221-228
Number of pages8
JournalMutation Research/Genetic Toxicology
Volume77
Issue number3
DOIs
StatePublished - 1980

Fingerprint

Mutagenesis
Salmonella
Benzo(a)pyrene
Microsomes
Metabolism
Assays
Metabolites
Kinetics
Vitamin K 3
Enzyme kinetics
Methylcholanthrene
Salmonella typhimurium
Rats
Liver
Liquids

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

Kinetics of benzo[a]pyrene induced mutagenesis in a highly sensitive Salmonella/microsome assay. / Unger, J.; Guttenplan, Joseph.

In: Mutation Research/Genetic Toxicology, Vol. 77, No. 3, 1980, p. 221-228.

Research output: Contribution to journalArticle

@article{7c99828be5ce47eca69dc9a6d32ddc83,
title = "Kinetics of benzo[a]pyrene induced mutagenesis in a highly sensitive Salmonella/microsome assay",
abstract = "The kinetics of benzo[a]pyrene induced mutagenesis in Salmonella typhimurium TA98 and TA100 were studied using a liquid-phase assay and were compared to kinetics of metabolism of benzo[a]pyrene in the same system. Mutagenesis was terminated by the addition of pyrene + menadione at selected times. Under conditions of the assay using hepatic microsomes from 3-methylcholanthrene pretreated rats, metabolism of benzo[a]pyrene was over 50{\%} complete in 5 min, and metabolism of initial metabolites became appreciable after this time. The revertant frequency (revertants/108 survivors) and the production of tertiary metabolites increased sharply after 5 min and leveled off at 40 min.",
author = "J. Unger and Joseph Guttenplan",
year = "1980",
doi = "10.1016/0165-1218(80)90054-3",
language = "English (US)",
volume = "77",
pages = "221--228",
journal = "Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure",
issn = "0165-1218",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Kinetics of benzo[a]pyrene induced mutagenesis in a highly sensitive Salmonella/microsome assay

AU - Unger, J.

AU - Guttenplan, Joseph

PY - 1980

Y1 - 1980

N2 - The kinetics of benzo[a]pyrene induced mutagenesis in Salmonella typhimurium TA98 and TA100 were studied using a liquid-phase assay and were compared to kinetics of metabolism of benzo[a]pyrene in the same system. Mutagenesis was terminated by the addition of pyrene + menadione at selected times. Under conditions of the assay using hepatic microsomes from 3-methylcholanthrene pretreated rats, metabolism of benzo[a]pyrene was over 50% complete in 5 min, and metabolism of initial metabolites became appreciable after this time. The revertant frequency (revertants/108 survivors) and the production of tertiary metabolites increased sharply after 5 min and leveled off at 40 min.

AB - The kinetics of benzo[a]pyrene induced mutagenesis in Salmonella typhimurium TA98 and TA100 were studied using a liquid-phase assay and were compared to kinetics of metabolism of benzo[a]pyrene in the same system. Mutagenesis was terminated by the addition of pyrene + menadione at selected times. Under conditions of the assay using hepatic microsomes from 3-methylcholanthrene pretreated rats, metabolism of benzo[a]pyrene was over 50% complete in 5 min, and metabolism of initial metabolites became appreciable after this time. The revertant frequency (revertants/108 survivors) and the production of tertiary metabolites increased sharply after 5 min and leveled off at 40 min.

UR - http://www.scopus.com/inward/record.url?scp=0018842232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018842232&partnerID=8YFLogxK

U2 - 10.1016/0165-1218(80)90054-3

DO - 10.1016/0165-1218(80)90054-3

M3 - Article

VL - 77

SP - 221

EP - 228

JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

SN - 0165-1218

IS - 3

ER -