Iterative Role of Notch Signaling in Spinal Motor Neuron Diversification

G. Christopher Tan, Esteban Mazzoni, Hynek Wichterle

Research output: Contribution to journalArticle

Abstract

The motor neuron progenitor domain in the ventral spinal cord gives rise to multiple subtypes of motor neurons and glial cells. Here, we examine whether progenitors found in this domain are multipotent and which signals contribute to their cell-type-specific differentiation. Using an in vitro neural differentiation model, we demonstrate that motor neuron progenitor differentiation is iteratively controlled by Notch signaling. First, Notch controls the timing of motor neuron genesis by repressing Neurogenin 2 (Ngn2) and maintaining Olig2-positive progenitors in a proliferative state. Second, in an Ngn2-independent manner, Notch contributes to the specification of median versus hypaxial motor column identity and lateral versus medial divisional identity of limb-innervating motor neurons. Thus, motor neuron progenitors are multipotent, and their diversification is controlled by Notch signaling that iteratively increases cellular diversity arising from a single neural progenitor domain. Tan et al. have found an iterative role for Notch signaling during motor neuron differentiation. Notch controls the timing of motor neuron genesis by suppressing Ngn2. Notch is subsequently required in an Ngn2-independent manner for the specification of median motor column identity over hypaxial and lateral motor neuron subtype identities.

Original languageEnglish (US)
JournalCell Reports
DOIs
StateAccepted/In press - Sep 16 2014

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Motor Neurons
Neurons
Specifications
Neuroglia
Cell Differentiation
Spinal Cord
Extremities

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Iterative Role of Notch Signaling in Spinal Motor Neuron Diversification. / Tan, G. Christopher; Mazzoni, Esteban; Wichterle, Hynek.

In: Cell Reports, 16.09.2014.

Research output: Contribution to journalArticle

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