Abstract
The motor neuron progenitor domain in the ventral spinal cord gives rise to multiple subtypes of motor neurons and glial cells. Here, we examine whether progenitors found in this domain are multipotent and which signals contribute to their cell-type-specific differentiation. Using an in vitro neural differentiation model, we demonstrate that motor neuron progenitor differentiation is iteratively controlled by Notch signaling. First, Notch controls the timing of motor neuron genesis by repressing Neurogenin 2 (Ngn2) and maintaining Olig2-positive progenitors in a proliferative state. Second, in an Ngn2-independent manner, Notch contributes to the specification of median versus hypaxial motor column identity and lateral versus medial divisional identity of limb-innervating motor neurons. Thus, motor neuron progenitors are multipotent, and their diversification is controlled by Notch signaling that iteratively increases cellular diversity arising from a single neural progenitor domain. Tan et al. have found an iterative role for Notch signaling during motor neuron differentiation. Notch controls the timing of motor neuron genesis by suppressing Ngn2. Notch is subsequently required in an Ngn2-independent manner for the specification of median motor column identity over hypaxial and lateral motor neuron subtype identities.
| Original language | English (US) |
|---|---|
| Journal | Cell Reports |
| DOIs | |
| State | Accepted/In press - Sep 16 2014 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Iterative Role of Notch Signaling in Spinal Motor Neuron Diversification. / Tan, G. Christopher; Mazzoni, Esteban; Wichterle, Hynek.
In: Cell Reports, 16.09.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Iterative Role of Notch Signaling in Spinal Motor Neuron Diversification
AU - Tan, G. Christopher
AU - Mazzoni, Esteban
AU - Wichterle, Hynek
PY - 2014/9/16
Y1 - 2014/9/16
N2 - The motor neuron progenitor domain in the ventral spinal cord gives rise to multiple subtypes of motor neurons and glial cells. Here, we examine whether progenitors found in this domain are multipotent and which signals contribute to their cell-type-specific differentiation. Using an in vitro neural differentiation model, we demonstrate that motor neuron progenitor differentiation is iteratively controlled by Notch signaling. First, Notch controls the timing of motor neuron genesis by repressing Neurogenin 2 (Ngn2) and maintaining Olig2-positive progenitors in a proliferative state. Second, in an Ngn2-independent manner, Notch contributes to the specification of median versus hypaxial motor column identity and lateral versus medial divisional identity of limb-innervating motor neurons. Thus, motor neuron progenitors are multipotent, and their diversification is controlled by Notch signaling that iteratively increases cellular diversity arising from a single neural progenitor domain. Tan et al. have found an iterative role for Notch signaling during motor neuron differentiation. Notch controls the timing of motor neuron genesis by suppressing Ngn2. Notch is subsequently required in an Ngn2-independent manner for the specification of median motor column identity over hypaxial and lateral motor neuron subtype identities.
AB - The motor neuron progenitor domain in the ventral spinal cord gives rise to multiple subtypes of motor neurons and glial cells. Here, we examine whether progenitors found in this domain are multipotent and which signals contribute to their cell-type-specific differentiation. Using an in vitro neural differentiation model, we demonstrate that motor neuron progenitor differentiation is iteratively controlled by Notch signaling. First, Notch controls the timing of motor neuron genesis by repressing Neurogenin 2 (Ngn2) and maintaining Olig2-positive progenitors in a proliferative state. Second, in an Ngn2-independent manner, Notch contributes to the specification of median versus hypaxial motor column identity and lateral versus medial divisional identity of limb-innervating motor neurons. Thus, motor neuron progenitors are multipotent, and their diversification is controlled by Notch signaling that iteratively increases cellular diversity arising from a single neural progenitor domain. Tan et al. have found an iterative role for Notch signaling during motor neuron differentiation. Notch controls the timing of motor neuron genesis by suppressing Ngn2. Notch is subsequently required in an Ngn2-independent manner for the specification of median motor column identity over hypaxial and lateral motor neuron subtype identities.
UR - http://www.scopus.com/inward/record.url?scp=84978910369&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978910369&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2016.06.067
DO - 10.1016/j.celrep.2016.06.067
M3 - Article
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
ER -