Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development

Lionel Christiaen, Eileen Wagner, Weiyang Shi, Michael Levine

Research output: Contribution to journalArticle

Abstract

This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trate amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.

Original languageEnglish (US)
JournalCold Spring Harbor Protocols
Volume4
Issue number12
DOIs
StatePublished - 2009

Fingerprint

Morpholinos
Soaps (detergents)
Fertilization
Germ Cells
Oceans and Seas
Detergents
Contacts (fluid mechanics)
Water quality
Embryonic Structures
DNA
Soaps
Electroporation
Water Quality
Microinjections
Eggs
In Situ Hybridization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development. / Christiaen, Lionel; Wagner, Eileen; Shi, Weiyang; Levine, Michael.

In: Cold Spring Harbor Protocols, Vol. 4, No. 12, 2009.

Research output: Contribution to journalArticle

@article{aeaba8dee53e44b1afe06abfe9ce33e3,
title = "Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development",
abstract = "This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trate amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.",
author = "Lionel Christiaen and Eileen Wagner and Weiyang Shi and Michael Levine",
year = "2009",
doi = "10.1101/pdb.prot5344",
language = "English (US)",
volume = "4",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
publisher = "Cold Spring Harbor Laboratory Press",
number = "12",

}

TY - JOUR

T1 - Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development

AU - Christiaen, Lionel

AU - Wagner, Eileen

AU - Shi, Weiyang

AU - Levine, Michael

PY - 2009

Y1 - 2009

N2 - This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trate amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.

AB - This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trate amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.

UR - http://www.scopus.com/inward/record.url?scp=72149087973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72149087973&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot5344

DO - 10.1101/pdb.prot5344

M3 - Article

VL - 4

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 12

ER -