Isolation of dominant XO-feminizing mutations in Caenorhabditis elegans

New regulatory tra alleles and an X chromosome duplication with implications for primary sex determination

J. Hodgkin, Donna Albertson

Research output: Contribution to journalArticle

Abstract

A strain of Caenorhabditis elegans was constructed that permits selection of dominant or sex-linked mutations that transform XO animals (normally male) into fertile females, using a feminizing mutation. tra-2(e2046gf), which by itself does not sexually transform XO males. Twenty-three mutations were isolated after chemical mutagenesis and found to fall into both expected classes (four dominant tra-I mutations and eight recessive xol-1 mutations) and novel classes. The novel mutations include 10 second-site mutations of tra-2, which are called eg mutations, for enhanced gain-of-function. The tra- 2(gf, eg) alleles lead to complete dominant transformation of XO animals from fertile male into fertile female. Also isolated was a duplication of the left end of the X chromosome, eDp26, which has dominant XO lethal and feminizing properties, unlike all previously isolated duplications of the X chromosome. The properties of eDp26 indicate that it carries copies of one or more numerator elements, which act as part of the primary sex-determination signal, the X:A ratio. The eDp26 duplication is attached to the left tip of the X chromosome in inverted orientation and consequently can be used to generate unstable attached-X chromosomes.

Original languageEnglish (US)
Pages (from-to)527-542
Number of pages16
JournalGenetics
Volume141
Issue number2
StatePublished - 1995

Fingerprint

Chromosome Duplication
Caenorhabditis elegans
X Chromosome
Alleles
Mutation
Mutagenesis

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

@article{4c6cf693f8314a02829866dee190a3d6,
title = "Isolation of dominant XO-feminizing mutations in Caenorhabditis elegans: New regulatory tra alleles and an X chromosome duplication with implications for primary sex determination",
abstract = "A strain of Caenorhabditis elegans was constructed that permits selection of dominant or sex-linked mutations that transform XO animals (normally male) into fertile females, using a feminizing mutation. tra-2(e2046gf), which by itself does not sexually transform XO males. Twenty-three mutations were isolated after chemical mutagenesis and found to fall into both expected classes (four dominant tra-I mutations and eight recessive xol-1 mutations) and novel classes. The novel mutations include 10 second-site mutations of tra-2, which are called eg mutations, for enhanced gain-of-function. The tra- 2(gf, eg) alleles lead to complete dominant transformation of XO animals from fertile male into fertile female. Also isolated was a duplication of the left end of the X chromosome, eDp26, which has dominant XO lethal and feminizing properties, unlike all previously isolated duplications of the X chromosome. The properties of eDp26 indicate that it carries copies of one or more numerator elements, which act as part of the primary sex-determination signal, the X:A ratio. The eDp26 duplication is attached to the left tip of the X chromosome in inverted orientation and consequently can be used to generate unstable attached-X chromosomes.",
author = "J. Hodgkin and Donna Albertson",
year = "1995",
language = "English (US)",
volume = "141",
pages = "527--542",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "2",

}

TY - JOUR

T1 - Isolation of dominant XO-feminizing mutations in Caenorhabditis elegans

T2 - New regulatory tra alleles and an X chromosome duplication with implications for primary sex determination

AU - Hodgkin, J.

AU - Albertson, Donna

PY - 1995

Y1 - 1995

N2 - A strain of Caenorhabditis elegans was constructed that permits selection of dominant or sex-linked mutations that transform XO animals (normally male) into fertile females, using a feminizing mutation. tra-2(e2046gf), which by itself does not sexually transform XO males. Twenty-three mutations were isolated after chemical mutagenesis and found to fall into both expected classes (four dominant tra-I mutations and eight recessive xol-1 mutations) and novel classes. The novel mutations include 10 second-site mutations of tra-2, which are called eg mutations, for enhanced gain-of-function. The tra- 2(gf, eg) alleles lead to complete dominant transformation of XO animals from fertile male into fertile female. Also isolated was a duplication of the left end of the X chromosome, eDp26, which has dominant XO lethal and feminizing properties, unlike all previously isolated duplications of the X chromosome. The properties of eDp26 indicate that it carries copies of one or more numerator elements, which act as part of the primary sex-determination signal, the X:A ratio. The eDp26 duplication is attached to the left tip of the X chromosome in inverted orientation and consequently can be used to generate unstable attached-X chromosomes.

AB - A strain of Caenorhabditis elegans was constructed that permits selection of dominant or sex-linked mutations that transform XO animals (normally male) into fertile females, using a feminizing mutation. tra-2(e2046gf), which by itself does not sexually transform XO males. Twenty-three mutations were isolated after chemical mutagenesis and found to fall into both expected classes (four dominant tra-I mutations and eight recessive xol-1 mutations) and novel classes. The novel mutations include 10 second-site mutations of tra-2, which are called eg mutations, for enhanced gain-of-function. The tra- 2(gf, eg) alleles lead to complete dominant transformation of XO animals from fertile male into fertile female. Also isolated was a duplication of the left end of the X chromosome, eDp26, which has dominant XO lethal and feminizing properties, unlike all previously isolated duplications of the X chromosome. The properties of eDp26 indicate that it carries copies of one or more numerator elements, which act as part of the primary sex-determination signal, the X:A ratio. The eDp26 duplication is attached to the left tip of the X chromosome in inverted orientation and consequently can be used to generate unstable attached-X chromosomes.

UR - http://www.scopus.com/inward/record.url?scp=0029147127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029147127&partnerID=8YFLogxK

M3 - Article

VL - 141

SP - 527

EP - 542

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 2

ER -