Isolation, culture and characterization of epithelial cells derived from rat ventral prostate

W. H J Douglas, Louis Terracio, H. Glass

Research output: Contribution to journalArticle

Abstract

Epithelial-cell enriched primary cultures have been established from rat ventral prostate (RVP). Minced ventral prostates were dissociated with 0.5% collagenase in F12K tissue culture medium containing 1% fetal bovine serum. This treatment resulted in the gradual removal of stromal elements from the base of the epithelial cells. After 60 minutes of digestion the aggregates of epithelial cells were washed and plated at high density in F12K plus 10% horse serum. After 48 hours in vitro the unattached cells were removed from the culture dishes, washed, and reinoculated into new culture vessels containing fresh medium. After 96 hours in vitro, the aggregates had attached to the culture vessels and spread out to yield discrete patches of epithelial cells. By 144 hours in vitro the patches of cells had grown and coalesced to form a semi-confluent monolayer of epithelial cells. Ultrastructural examination of these cultures indicated that adjacent cells were joined by desmosomes and tight junctions and had formed 'lumen-like structures' into which projected microvilli. In addition, the cells contained secretory granules and tonofilaments, giving them a morphological appearance similar to prostate epithelial cells in the intact organ. The primary cultures also retained histochemical activities for acid phosphatase, β-glucuronidase, and succinic dehydrogenase that were similar to the intact organ.

Original languageEnglish (US)
Pages (from-to)239-256
Number of pages18
JournalAnatomical Record
Volume197
Issue number2
StatePublished - 1980

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Prostate
epithelial cells
Epithelial Cells
rats
cell aggregates
serum
vessel
succinate dehydrogenase (quinone)
cells
desmosomes
Desmosomes
horse
collagenase
tight junctions
Succinate Dehydrogenase
Intermediate Filaments
phosphatase
Tight Junctions
secretory granules
Glucuronidase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Isolation, culture and characterization of epithelial cells derived from rat ventral prostate. / Douglas, W. H J; Terracio, Louis; Glass, H.

In: Anatomical Record, Vol. 197, No. 2, 1980, p. 239-256.

Research output: Contribution to journalArticle

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