Iso-lines and inbred-lines confirmed loci that underlie resistance from cultivar 'Hartwig' to three soybean cyst nematode populations

Samreen Kazi, J. Shultz, J. Afzal, Rizwan Hashmi, Mohammed Jasim, Jason Bond, Prakash R. Arelli, David A. Lightfoot

Research output: Contribution to journalArticle

Abstract

Soybean [Glycine max (L.) Merr.] cultivars varied in their resistance to different populations of the soybean cyst nematode (SCN), Heterodera glycines, called HG Types. The rhg1 locus on linkage group G was necessary for resistance to all HG types. However, the loci for resistance to H. glycines HG Type 1.3- (race 14) and HG Type 1.2.5- (race 2) of the soybean cyst nematode have varied in their reported locations. The aims were to compare the inheritance of resistance to three nematode HG Types in a population segregating for resistance to SCN and to identify the underlying quantitative trait loci (QTL). 'Hartwig', a soybean cultivar resistant to most SCN HG Types, was crossed with the susceptible cultivar 'Flyer'. A total of 92 F5-derived recombinant inbred lines (RILs; or inbred lines) and 144 molecular markers were used for map development. The rhg1 associated QTL found in earlier studies were confirmed and shown to underlie resistance to all three HG Types in RILs (Satt309; HG Type 0, P = 0.0001 R2 = 22%; Satt275; HG Type 1. 3, P = 0. 001, R2 = 14%) and near isogeneic lines (NILs; or iso-lines; Satt309; HG Type 1. 2.5-, P = 0.001 R2 = 24%). A new QTL underlying resistance to HG Type 1.2.5- was detected on LG D2 (Satt574; P = 0. 001, R2 = 11%) among 14 RILs resistant to the other HG types. The locus was confirmed in a small NIL population consisting of 60 plants of ten genotypes (P = 0.04). This QTL (cqSCN-005) is located in an interval previously associated with resistance to both SDS leaf scorch from 'Pyramid' and 'Ripley' (cqSDS-001) and SCN HG Type 1.3- from Hartwig and Pyramid. The QTL detected will allow marker assisted selection for multigenic resistance to complex nematode populations in combination with sudden death syndrome resistance (SDS) and other agronomic traits.

Original languageEnglish (US)
Pages (from-to)633-644
Number of pages12
JournalTheoretical and Applied Genetics
Volume120
Issue number3
DOIs
StatePublished - Jan 1 2010

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Heterodera glycines
Soybeans
inbred lines
Cysts
Quantitative Trait Loci
loci
quantitative trait loci
cultivars
Population
Sudden Death
Glycine
Nematoda
soybeans
death
scorch
isogenic lines
linkage groups
marker-assisted selection
agronomic traits
Glycine max

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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Iso-lines and inbred-lines confirmed loci that underlie resistance from cultivar 'Hartwig' to three soybean cyst nematode populations. / Kazi, Samreen; Shultz, J.; Afzal, J.; Hashmi, Rizwan; Jasim, Mohammed; Bond, Jason; Arelli, Prakash R.; Lightfoot, David A.

In: Theoretical and Applied Genetics, Vol. 120, No. 3, 01.01.2010, p. 633-644.

Research output: Contribution to journalArticle

Kazi, Samreen ; Shultz, J. ; Afzal, J. ; Hashmi, Rizwan ; Jasim, Mohammed ; Bond, Jason ; Arelli, Prakash R. ; Lightfoot, David A. / Iso-lines and inbred-lines confirmed loci that underlie resistance from cultivar 'Hartwig' to three soybean cyst nematode populations. In: Theoretical and Applied Genetics. 2010 ; Vol. 120, No. 3. pp. 633-644.
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abstract = "Soybean [Glycine max (L.) Merr.] cultivars varied in their resistance to different populations of the soybean cyst nematode (SCN), Heterodera glycines, called HG Types. The rhg1 locus on linkage group G was necessary for resistance to all HG types. However, the loci for resistance to H. glycines HG Type 1.3- (race 14) and HG Type 1.2.5- (race 2) of the soybean cyst nematode have varied in their reported locations. The aims were to compare the inheritance of resistance to three nematode HG Types in a population segregating for resistance to SCN and to identify the underlying quantitative trait loci (QTL). 'Hartwig', a soybean cultivar resistant to most SCN HG Types, was crossed with the susceptible cultivar 'Flyer'. A total of 92 F5-derived recombinant inbred lines (RILs; or inbred lines) and 144 molecular markers were used for map development. The rhg1 associated QTL found in earlier studies were confirmed and shown to underlie resistance to all three HG Types in RILs (Satt309; HG Type 0, P = 0.0001 R2 = 22{\%}; Satt275; HG Type 1. 3, P = 0. 001, R2 = 14{\%}) and near isogeneic lines (NILs; or iso-lines; Satt309; HG Type 1. 2.5-, P = 0.001 R2 = 24{\%}). A new QTL underlying resistance to HG Type 1.2.5- was detected on LG D2 (Satt574; P = 0. 001, R2 = 11{\%}) among 14 RILs resistant to the other HG types. The locus was confirmed in a small NIL population consisting of 60 plants of ten genotypes (P = 0.04). This QTL (cqSCN-005) is located in an interval previously associated with resistance to both SDS leaf scorch from 'Pyramid' and 'Ripley' (cqSDS-001) and SCN HG Type 1.3- from Hartwig and Pyramid. The QTL detected will allow marker assisted selection for multigenic resistance to complex nematode populations in combination with sudden death syndrome resistance (SDS) and other agronomic traits.",
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AU - Hashmi, Rizwan

AU - Jasim, Mohammed

AU - Bond, Jason

AU - Arelli, Prakash R.

AU - Lightfoot, David A.

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