Investigation of biosensing bias with single cells located in rectangular microwells

Ayoola T. Brimmo, Pavithra Sukumar, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here, we numerically justify precise positioning of single cells in rectangular microwells for accurate secretory protein sensing, and report a microfluidic single cell per microwell positioning device. Our results show significant time and concentration bias in multiplexed cytokine sensors based on the position of the single cells relative to the sensor. By combining air plugs with immunochemistry in a microfluidic chip, single cells were successfully positioned at the center of 450 microwells. This device could potentially play a vital role in acquiring precise sensitivity and specificity in secretory protein temporal sensing at the single cell level.

Original languageEnglish (US)
Title of host publication2019 Advances in Science and Engineering Technology International Conferences, ASET 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538682715
DOIs
StatePublished - May 14 2019
Event2019 Advances in Science and Engineering Technology International Conferences, ASET 2019 - Dubai, United Arab Emirates
Duration: Mar 26 2019Apr 10 2019

Publication series

Name2019 Advances in Science and Engineering Technology International Conferences, ASET 2019

Conference

Conference2019 Advances in Science and Engineering Technology International Conferences, ASET 2019
CountryUnited Arab Emirates
CityDubai
Period3/26/194/10/19

Keywords

  • Microfluidics
  • air plugs
  • cytokines
  • rectangular microwells
  • secretory proteins
  • sensing bias
  • single cells
  • temporal biosensing

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Artificial Intelligence
  • Computer Networks and Communications
  • Energy Engineering and Power Technology
  • Civil and Structural Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Ceramics and Composites

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  • Cite this

    Brimmo, A. T., Sukumar, P., & Qasaimeh, M. A. (2019). Investigation of biosensing bias with single cells located in rectangular microwells. In 2019 Advances in Science and Engineering Technology International Conferences, ASET 2019 [8714469] (2019 Advances in Science and Engineering Technology International Conferences, ASET 2019). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICASET.2019.8714469