Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord

Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Ascidian embryos have been employed as model systems for studies of developmental biology for well over a century, owing to their desirable blend of experimental advantages, which include their rapid development, traceable cell lineage, and evolutionarily conserved morphogenetic movements. Two decades ago, the development of a streamlined electroporation method drastically reduced the time and cost of transgenic experiments, and, along with the elucidation of the complete genomic sequences of several ascidian species, propelled these simple chordates to the forefront of the model organisms available for studies of regulation of gene expression. Numerous ascidian sequences with tissue-specific enhancer activity were isolated and rapidly characterized through systematic in vivo experiments that would require several weeks in most other model systems. These cis-regulatory sequences include a large collection of notochord enhancers, which have been used to visualize notochord development in vivo, to generate mutant phenotypes, and to knock down genes of interest. Moreover, their detailed characterization has allowed the reconstruction of different branches of the notochord gene regulatory network. This chapter describes how the use of transgenic techniques has rendered the ascidian Ciona a competitive model organism for studies of notochord development, evolution, and gene regulation.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages81-99
Number of pages19
DOIs
StatePublished - Jan 1 2018

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1029
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Fingerprint

Notochord
Urochordata
Gene expression
Genes
Gene Expression
Experiments
Tissue
Chordata
Gene Knockdown Techniques
Developmental Biology
Electroporation
Gene Regulatory Networks
Gene Expression Regulation
Cell Lineage
Costs
Embryonic Structures
Phenotype
Costs and Cost Analysis

Keywords

  • Ascidian
  • Brachyury
  • Ciona
  • cis-Regulatory Module
  • Electroporation
  • Enhancer
  • Notochord
  • T-Box
  • Tbx2/3
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Maguire, J. E., Pandey, A., Wu, Y., & Di Gregorio, A. (2018). Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord. In Advances in Experimental Medicine and Biology (pp. 81-99). (Advances in Experimental Medicine and Biology; Vol. 1029). Springer New York LLC. https://doi.org/10.1007/978-981-10-7545-2_8

Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord. / Maguire, Julie E.; Pandey, Aakarsha; Wu, Yushi; Di Gregorio, Anna.

Advances in Experimental Medicine and Biology. Springer New York LLC, 2018. p. 81-99 (Advances in Experimental Medicine and Biology; Vol. 1029).

Research output: Chapter in Book/Report/Conference proceedingChapter

Maguire, JE, Pandey, A, Wu, Y & Di Gregorio, A 2018, Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord. in Advances in Experimental Medicine and Biology. Advances in Experimental Medicine and Biology, vol. 1029, Springer New York LLC, pp. 81-99. https://doi.org/10.1007/978-981-10-7545-2_8
Maguire JE, Pandey A, Wu Y, Di Gregorio A. Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord. In Advances in Experimental Medicine and Biology. Springer New York LLC. 2018. p. 81-99. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-981-10-7545-2_8
Maguire, Julie E. ; Pandey, Aakarsha ; Wu, Yushi ; Di Gregorio, Anna. / Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord. Advances in Experimental Medicine and Biology. Springer New York LLC, 2018. pp. 81-99 (Advances in Experimental Medicine and Biology).
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