Intrinsic cleavage of RNA polymerase II adopts a nucleobase-independent mechanism assisted by transcript phosphate

Carmen Ka Man Tse, Jun Xu, Liang Xu, Fu Kit Sheong, Shenglong Wang, Hoi Yee Chow, Xin Gao, Xuechen Li, Peter Pak Hang Cheung, Dong Wang, Yingkai Zhang, Xuhui Huang

Research output: Contribution to journalArticle

Abstract

RNA polymerase II (Pol II) utilizes the same active site for polymerization and intrinsic cleavage. Pol II proofreads the nascent transcript via its intrinsic nuclease activity to maintain high transcriptional fidelity critical for cell growth and viability. The detailed catalytic mechanism of intrinsic cleavage remains unknown. Here, we combined ab initio quantum mechanics/molecular mechanics studies and biochemical cleavage assays to show that Pol II utilizes downstream phosphate oxygen to activate the attacking nucleophile in hydrolysis, while the newly formed 3′-end is protonated through active-site water without a defined general acid. Experimentally, alteration of downstream phosphate oxygen either by 2′-5′ sugar linkage or stereo-specific thio-substitution of phosphate oxygen drastically reduced cleavage rate. We showed by N7-modification that guanine nucleobase is not directly involved as an acid–base catalyst. Our proposed mechanism provides important insights into the intrinsic transcriptional cleavage reaction, an essential step in transcriptional fidelity control.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalNature Catalysis
Volume2
Issue number3
DOIs
StatePublished - Mar 1 2019

Fingerprint

RNA Polymerase II
RNA
Phosphates
Oxygen
Mechanics
Catalytic Domain
Nucleophiles
Molecular mechanics
Quantum theory
Guanine
Cell growth
Sugars
Polymerization
Hydrolysis
Assays
Cell Survival
Substitution reactions
Catalysts
Acids
Water

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

Cite this

Intrinsic cleavage of RNA polymerase II adopts a nucleobase-independent mechanism assisted by transcript phosphate. / Tse, Carmen Ka Man; Xu, Jun; Xu, Liang; Sheong, Fu Kit; Wang, Shenglong; Chow, Hoi Yee; Gao, Xin; Li, Xuechen; Cheung, Peter Pak Hang; Wang, Dong; Zhang, Yingkai; Huang, Xuhui.

In: Nature Catalysis, Vol. 2, No. 3, 01.03.2019, p. 228-235.

Research output: Contribution to journalArticle

Tse, CKM, Xu, J, Xu, L, Sheong, FK, Wang, S, Chow, HY, Gao, X, Li, X, Cheung, PPH, Wang, D, Zhang, Y & Huang, X 2019, 'Intrinsic cleavage of RNA polymerase II adopts a nucleobase-independent mechanism assisted by transcript phosphate', Nature Catalysis, vol. 2, no. 3, pp. 228-235. https://doi.org/10.1038/s41929-019-0227-5
Tse, Carmen Ka Man ; Xu, Jun ; Xu, Liang ; Sheong, Fu Kit ; Wang, Shenglong ; Chow, Hoi Yee ; Gao, Xin ; Li, Xuechen ; Cheung, Peter Pak Hang ; Wang, Dong ; Zhang, Yingkai ; Huang, Xuhui. / Intrinsic cleavage of RNA polymerase II adopts a nucleobase-independent mechanism assisted by transcript phosphate. In: Nature Catalysis. 2019 ; Vol. 2, No. 3. pp. 228-235.
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