Intracellular pH regulates the production of different oxygen metabolites in neutrophils: Effects of organic acids produced by anaerobic bacteria

M. Tonetti, A. Cavallero, G. A. Botta, Richard Niederman, C. Eftimiadi

Research output: Contribution to journalArticle

Abstract

The effects of short chain carboxylic acids (SCCA), namely succinic, butyric, and iso-butyric, on neutrophil metabolic activation were assessed. SCCA-induced a significant decrease in O 2· - recovery and chemiluminescent response in neutrophils activated with the diacylglycerol analog tetradecanoyl-phorbol-acetate (TPA). SCCA did not alter O 2 consumption, H 2O 2 production, or the calorimetrically determined energy expenditure occurring during the metabolic burst. SCCA also induced a significant acidification of intracellular pH (pH(i)). These results are consistent with an increased divalent versus univalent O 2 reduction performed by the NADPH oxidase at a more acidic intracellular pH.

Original languageEnglish (US)
Pages (from-to)180-188
Number of pages9
JournalJournal of Leukocyte Biology
Volume49
Issue number2
StatePublished - 1991

Fingerprint

Anaerobic Bacteria
Carboxylic Acids
Neutrophils
Oxygen
Acids
Neutrophil Activation
NADPH Oxidase
Diglycerides
Succinic Acid
Energy Metabolism
Acetates

Keywords

  • butyrates
  • calorimetry
  • hydrogen ion concentration
  • metabolism
  • oxygen radicals
  • phagocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Intracellular pH regulates the production of different oxygen metabolites in neutrophils : Effects of organic acids produced by anaerobic bacteria. / Tonetti, M.; Cavallero, A.; Botta, G. A.; Niederman, Richard; Eftimiadi, C.

In: Journal of Leukocyte Biology, Vol. 49, No. 2, 1991, p. 180-188.

Research output: Contribution to journalArticle

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