Intracellular pH regulates the production of different oxygen metabolites in neutrophils: Effects of organic acids produced by anaerobic bacteria

M. Tonetti, A. Cavallero, G. A. Botta, R. Niederman, C. Eftimiadi

Research output: Contribution to journalArticle


The effects of short chain carboxylic acids (SCCA), namely succinic, butyric, and iso-butyric, on neutrophil metabolic activation were assessed. SCCA-induced a significant decrease in O2·- recovery and chemiluminescent response in neutrophils activated with the diacylglycerol analog tetradecanoyl-phorbol-acetate (TPA). SCCA did not alter O2 consumption, H2O2 production, or the calorimetrically determined energy expenditure occurring during the metabolic burst. SCCA also induced a significant acidification of intracellular pH (pH(i)). These results are consistent with an increased divalent versus univalent O2 reduction performed by the NADPH oxidase at a more acidic intracellular pH.

Original languageEnglish (US)
Pages (from-to)180-188
Number of pages9
JournalJournal of Leukocyte Biology
Issue number2
StatePublished - Jan 1 1991



  • butyrates
  • calorimetry
  • hydrogen ion concentration
  • metabolism
  • oxygen radicals
  • phagocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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