Intracellular fluid flow in rapidly moving cells

Kinneret Keren, Patricia T. Yam, Anika Kinkhabwala, Alex Mogilner, Julie A. Theriot

Research output: Contribution to journalArticle

Abstract

Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert quantum dots into the lamellipodia of fish epithelial keratocytes and analysed their distribution and motion. Our results indicate that fluid flow is directed from the cell body towards the leading edge in the cell frame of reference, at about 40% of cell speed. We propose that this forward-directed flow is driven by increased hydrostatic pressure generated at the rear of the cell by myosin contraction, and show that inhibition of myosin II activity by blebbistatin reverses the direction of fluid flow and leads to a decrease in keratocyte speed. We present a physical model for fluid pressure and flow in moving cells that quantitatively accounts for our experimental data.

Original languageEnglish (US)
Pages (from-to)1219-1224
Number of pages6
JournalNature Cell Biology
Volume11
Issue number10
DOIs
StatePublished - 2009

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Intracellular Fluid
Hydrodynamics
Myosin Type II
Quantum Dots
Pseudopodia
Hydrostatic Pressure
Myosins
Cell Movement
Actins
Fishes
Pressure

ASJC Scopus subject areas

  • Cell Biology

Cite this

Keren, K., Yam, P. T., Kinkhabwala, A., Mogilner, A., & Theriot, J. A. (2009). Intracellular fluid flow in rapidly moving cells. Nature Cell Biology, 11(10), 1219-1224. https://doi.org/10.1038/ncb1965

Intracellular fluid flow in rapidly moving cells. / Keren, Kinneret; Yam, Patricia T.; Kinkhabwala, Anika; Mogilner, Alex; Theriot, Julie A.

In: Nature Cell Biology, Vol. 11, No. 10, 2009, p. 1219-1224.

Research output: Contribution to journalArticle

Keren, K, Yam, PT, Kinkhabwala, A, Mogilner, A & Theriot, JA 2009, 'Intracellular fluid flow in rapidly moving cells', Nature Cell Biology, vol. 11, no. 10, pp. 1219-1224. https://doi.org/10.1038/ncb1965
Keren, Kinneret ; Yam, Patricia T. ; Kinkhabwala, Anika ; Mogilner, Alex ; Theriot, Julie A. / Intracellular fluid flow in rapidly moving cells. In: Nature Cell Biology. 2009 ; Vol. 11, No. 10. pp. 1219-1224.
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