Interleukin (IL)-12 receptor β1 or IL-12 receptor β2 deficiency in mice indicates that IL-12 and IL-23 are not essential for host recovery from viral encephalitis

Derek D C Ireland, Beth M. Palian, Carol Shoshkes Reiss

Research output: Contribution to journalArticle

Abstract

Vesicular stomatitis virus (VSV), a negative-sense, single-stranded RNA rhabdovirus, causes acute viral encephalitis when administered intranasally to mice. Interleukin-12 (IL-12) is a key pro-inflammatory cytokine that is produced largely by the antigen presenting cells (APC) and that bridges the innate and acquired immune responses. IL-12 is efficacious in enhancing recovery from VSV infection of the murine central nervous system. This effect is mediated by nitric oxide (NO) produced by the neuronal isoform of nitric oxide synthase (NOS-1), and is independent of the pro-inflammatory cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). These data implied a link between IL-12 and NOS-1. Here we investigate the role of the IL-12R during VSV pathogenesis, using IL-12Rβ2 and IL-12Rβ1-deficient mice. We showed that a deficiency in either IL-12Rβ2 or IL-12Rβ1 had no effect on the outcome of VSV infection of the CNS or on the clearance of VSV from the CNS. Furthermore, these data indicate that IL-23 is not acting redundantly in the absence of IL-12 during VSV-induced encephalitis.

Original languageEnglish (US)
Pages (from-to)397-402
Number of pages6
JournalViral Immunology
Volume18
Issue number2
DOIs
StatePublished - 2005

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Interleukin-12 Receptors
Viral Encephalitis
Interleukin-23
Vesicular Stomatitis
Interleukin-12
Interleukins
Virus Diseases
Viruses
Encephalitis Viruses
Rhabdoviridae
Cytokines
Nitric Oxide Synthase Type I
Antigen-Presenting Cells
Innate Immunity
Interferons
Nitric Oxide
Protein Isoforms
Central Nervous System
Tumor Necrosis Factor-alpha
RNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

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title = "Interleukin (IL)-12 receptor β1 or IL-12 receptor β2 deficiency in mice indicates that IL-12 and IL-23 are not essential for host recovery from viral encephalitis",
abstract = "Vesicular stomatitis virus (VSV), a negative-sense, single-stranded RNA rhabdovirus, causes acute viral encephalitis when administered intranasally to mice. Interleukin-12 (IL-12) is a key pro-inflammatory cytokine that is produced largely by the antigen presenting cells (APC) and that bridges the innate and acquired immune responses. IL-12 is efficacious in enhancing recovery from VSV infection of the murine central nervous system. This effect is mediated by nitric oxide (NO) produced by the neuronal isoform of nitric oxide synthase (NOS-1), and is independent of the pro-inflammatory cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). These data implied a link between IL-12 and NOS-1. Here we investigate the role of the IL-12R during VSV pathogenesis, using IL-12Rβ2 and IL-12Rβ1-deficient mice. We showed that a deficiency in either IL-12Rβ2 or IL-12Rβ1 had no effect on the outcome of VSV infection of the CNS or on the clearance of VSV from the CNS. Furthermore, these data indicate that IL-23 is not acting redundantly in the absence of IL-12 during VSV-induced encephalitis.",
author = "Ireland, {Derek D C} and Palian, {Beth M.} and Reiss, {Carol Shoshkes}",
year = "2005",
doi = "10.1089/vim.2005.18.397",
language = "English (US)",
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pages = "397--402",
journal = "Viral Immunology",
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T1 - Interleukin (IL)-12 receptor β1 or IL-12 receptor β2 deficiency in mice indicates that IL-12 and IL-23 are not essential for host recovery from viral encephalitis

AU - Ireland, Derek D C

AU - Palian, Beth M.

AU - Reiss, Carol Shoshkes

PY - 2005

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N2 - Vesicular stomatitis virus (VSV), a negative-sense, single-stranded RNA rhabdovirus, causes acute viral encephalitis when administered intranasally to mice. Interleukin-12 (IL-12) is a key pro-inflammatory cytokine that is produced largely by the antigen presenting cells (APC) and that bridges the innate and acquired immune responses. IL-12 is efficacious in enhancing recovery from VSV infection of the murine central nervous system. This effect is mediated by nitric oxide (NO) produced by the neuronal isoform of nitric oxide synthase (NOS-1), and is independent of the pro-inflammatory cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). These data implied a link between IL-12 and NOS-1. Here we investigate the role of the IL-12R during VSV pathogenesis, using IL-12Rβ2 and IL-12Rβ1-deficient mice. We showed that a deficiency in either IL-12Rβ2 or IL-12Rβ1 had no effect on the outcome of VSV infection of the CNS or on the clearance of VSV from the CNS. Furthermore, these data indicate that IL-23 is not acting redundantly in the absence of IL-12 during VSV-induced encephalitis.

AB - Vesicular stomatitis virus (VSV), a negative-sense, single-stranded RNA rhabdovirus, causes acute viral encephalitis when administered intranasally to mice. Interleukin-12 (IL-12) is a key pro-inflammatory cytokine that is produced largely by the antigen presenting cells (APC) and that bridges the innate and acquired immune responses. IL-12 is efficacious in enhancing recovery from VSV infection of the murine central nervous system. This effect is mediated by nitric oxide (NO) produced by the neuronal isoform of nitric oxide synthase (NOS-1), and is independent of the pro-inflammatory cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). These data implied a link between IL-12 and NOS-1. Here we investigate the role of the IL-12R during VSV pathogenesis, using IL-12Rβ2 and IL-12Rβ1-deficient mice. We showed that a deficiency in either IL-12Rβ2 or IL-12Rβ1 had no effect on the outcome of VSV infection of the CNS or on the clearance of VSV from the CNS. Furthermore, these data indicate that IL-23 is not acting redundantly in the absence of IL-12 during VSV-induced encephalitis.

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