Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides

A. Slama-Schwok, Nicholas Geacintov, V. Ibanez, N. E. Geacintov, T. Montenay-Garestier, J. M. Lehn, C. Hélène

Research output: Contribution to journalArticle

Abstract

The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.

Original languageEnglish (US)
Pages (from-to)3234-3242
Number of pages9
JournalBiochemistry
Volume28
Issue number8
StatePublished - 1989

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Polynucleotides
Nucleic Acids
Fluorescence
Linear Energy Transfer
DNA
Intercalation
Energy transfer
Coloring Agents
dimethyldiazaperopyrenium

ASJC Scopus subject areas

  • Biochemistry

Cite this

Slama-Schwok, A., Geacintov, N., Ibanez, V., Geacintov, N. E., Montenay-Garestier, T., Lehn, J. M., & Hélène, C. (1989). Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides. Biochemistry, 28(8), 3234-3242.

Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides. / Slama-Schwok, A.; Geacintov, Nicholas; Ibanez, V.; Geacintov, N. E.; Montenay-Garestier, T.; Lehn, J. M.; Hélène, C.

In: Biochemistry, Vol. 28, No. 8, 1989, p. 3234-3242.

Research output: Contribution to journalArticle

Slama-Schwok, A, Geacintov, N, Ibanez, V, Geacintov, NE, Montenay-Garestier, T, Lehn, JM & Hélène, C 1989, 'Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides', Biochemistry, vol. 28, no. 8, pp. 3234-3242.
Slama-Schwok A, Geacintov N, Ibanez V, Geacintov NE, Montenay-Garestier T, Lehn JM et al. Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides. Biochemistry. 1989;28(8):3234-3242.
Slama-Schwok, A. ; Geacintov, Nicholas ; Ibanez, V. ; Geacintov, N. E. ; Montenay-Garestier, T. ; Lehn, J. M. ; Hélène, C. / Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides. In: Biochemistry. 1989 ; Vol. 28, No. 8. pp. 3234-3242.
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