Interactions of recombinant HMGB proteins with branched RNA substrates

Anthony J. Bell, Seema Chauhan, Sarah A. Woodson, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

The high mobility group protein HMGB1 is a highly abundant chromosomal protein known to interact preferentially with DNA that is branched, bent or otherwise structurally altered. Biologically the protein is thought to facilitate promoter attachment by transcription factors. Recently, however, HMGB1 has been shown to have biological roles beyond that of an architectural DNA-binding protein. Here we investigate the binding interactions of recombinant HMGB1 proteins with two branched RNA's E. coli 5S rRNA and the group I intron ribozyme from Azoarcus pre-tRNAIle. Using competitive electrophoretic mobility and circular dichroism binding assays, we show that HMGB proteins bind both substrates with high affinity. We also report that a recombinant rat HMGB protein, rHMGB1b, inhibits RNA cleavage by the ribozyme. These results raise the possibility that HMGB proteins possess structure dependent RNA binding activity and can modulate RNA processing as well as transcription.

Original languageEnglish (US)
Pages (from-to)262-267
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number1
DOIs
StatePublished - Dec 5 2008

Fingerprint

HMGB Proteins
HMGB1 Protein
Recombinant proteins
Recombinant Proteins
RNA
Catalytic RNA
Azoarcus
RNA, Transfer, Ile
Substrates
RNA Cleavage
High Mobility Group Proteins
DNA-Binding Proteins
Circular Dichroism
Electrophoretic mobility
Introns
Dichroism
Proteins
Transcription Factors
Transcription
Escherichia coli

Keywords

  • DNA binding
  • Four-way junction DNA
  • High mobility group proteins
  • Ribozyme
  • RNA binding

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Interactions of recombinant HMGB proteins with branched RNA substrates. / Bell, Anthony J.; Chauhan, Seema; Woodson, Sarah A.; Kallenbach, Neville R.

In: Biochemical and Biophysical Research Communications, Vol. 377, No. 1, 05.12.2008, p. 262-267.

Research output: Contribution to journalArticle

Bell, Anthony J. ; Chauhan, Seema ; Woodson, Sarah A. ; Kallenbach, Neville R. / Interactions of recombinant HMGB proteins with branched RNA substrates. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 377, No. 1. pp. 262-267.
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