Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces

W. O. Soboyejo, B. Nemetski, S. Allameh, N. Marcantonio, C. Mercer, John Ricci

Research output: Contribution to journalArticle

Abstract

This paper presents the results of an experimental study of the interactions between MC3T3-E1 (mouse calvarian) cells and textured Ti6A14V surfaces, including surfaces produced by laser microgrooving; blasting with alumina particles; and polishing. The multiscale interactions between MC3T3-E1 cells and these textured surfaces are studied using a combination of optical scanning transmission electron microscopy and atomic force microscopy. The potential cytotoxic effects of microchemistry on cell-surface interactions also are considered in studies of cell spreading and orientation over 9-day periods. These studies show that cells on microgrooved Ti6A14V geometries that are 8 or 12 μm deep undergo contact guidance and limited cell spreading. Similar contact guidance is observed on the surfaces of diamond-polished surfaces on which nanoscale grooves are formed due to the scratching that occurs during polishing. In contrast, random cell orientations are observed on alumina-blasted Ti6A14V surfaces. The possible effects of surface topography are discussed for scar-tissue formation and improved cell-surface integration.

Original languageEnglish (US)
Pages (from-to)56-72
Number of pages17
JournalJournal of Biomedical Materials Research
Volume62
Issue number1
DOIs
StatePublished - 2002

Fingerprint

Aluminum Oxide
Polishing
Alumina
Diamond
titanium alloy (TiAl6V4)
Blasting
Surface topography
Atomic force microscopy
Diamonds
Tissue
Transmission electron microscopy
Scanning
Scanning electron microscopy
Geometry
Lasers

Keywords

  • Cell-surface interactions
  • Laser microgrooves
  • MC3T3-E1 cells
  • Surface textures
  • Ti6Al4V

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces. / Soboyejo, W. O.; Nemetski, B.; Allameh, S.; Marcantonio, N.; Mercer, C.; Ricci, John.

In: Journal of Biomedical Materials Research, Vol. 62, No. 1, 2002, p. 56-72.

Research output: Contribution to journalArticle

Soboyejo, WO, Nemetski, B, Allameh, S, Marcantonio, N, Mercer, C & Ricci, J 2002, 'Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces', Journal of Biomedical Materials Research, vol. 62, no. 1, pp. 56-72. https://doi.org/10.1002/jbm.10221
Soboyejo, W. O. ; Nemetski, B. ; Allameh, S. ; Marcantonio, N. ; Mercer, C. ; Ricci, John. / Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces. In: Journal of Biomedical Materials Research. 2002 ; Vol. 62, No. 1. pp. 56-72.
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