Interaction and structure induction of cell-penetrating peptides in the presence of phospholipid vesicles

Mazin Magzoub, Kalle Kilk, L. E.Göran Eriksson, Ülo Langel, Astrid Gräslund

Research output: Contribution to journalArticle

Abstract

Certain short peptides, which are able to translocate across cell membranes with a low lytic activity, can be useful as carriers (vectors) for hydrophilic molecules. We have studied three such cell penetrating peptides: pAntp ('penetratin'), pIsl and transportan. pAntp and pIsl originate from the third helix of homeodomain proteins (Antennapedia and Isl-1, respectively). Transportan is a synthetic chimera (galanin and mastoparan). The peptides in the presence of various phospholipid vesicles (neutral and charged) and SDS micelles have been characterized by spectroscopic methods (fluorescence, EPR and CD). The dynamics of pAntp were monitored using an N-terminal spin label. In aqueous solution, the CD spectra of the three peptides show secondary structures dominated by random coil. With phospholipid vesicles, neutral as well as negatively charged, transportan gives up to 60% α-helix. pAntp and pIsl bind significantly only to negatively charged vesicles with an induction of around 60% β-sheet-like secondary structure. With all three peptides, SDS micelles stabilize a high degree of α-helical structure. We conclude that the exact nature of any secondary structure induced by the membrane model systems is not directly correlated with the common transport property of these translocating peptides.

Original languageEnglish (US)
Pages (from-to)77-89
Number of pages13
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1512
Issue number1
DOIs
StatePublished - May 2 2001

Fingerprint

Cell-Penetrating Peptides
Phospholipids
Peptides
Micelles
Antennapedia Homeodomain Protein
Spin Labels
Galanin
Cell membranes
Transport properties
Paramagnetic resonance
Fluorescence
Cell Membrane
Membranes
Molecules
transportan

Keywords

  • Homeo-peptide
  • Interaction
  • Penetratin
  • Phospholipid vesicle
  • Secondary structure
  • Transportan

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Interaction and structure induction of cell-penetrating peptides in the presence of phospholipid vesicles. / Magzoub, Mazin; Kilk, Kalle; Eriksson, L. E.Göran; Langel, Ülo; Gräslund, Astrid.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1512, No. 1, 02.05.2001, p. 77-89.

Research output: Contribution to journalArticle

Magzoub, Mazin ; Kilk, Kalle ; Eriksson, L. E.Göran ; Langel, Ülo ; Gräslund, Astrid. / Interaction and structure induction of cell-penetrating peptides in the presence of phospholipid vesicles. In: Biochimica et Biophysica Acta - Biomembranes. 2001 ; Vol. 1512, No. 1. pp. 77-89.
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