Integrin-extracellular matrix interactions in connective tissue remodeling and osteoblast differentiation.

R. K. Globus, Amr Moursi, D. Zimmerman, J. Lull, C. Damsky

Research output: Contribution to journalArticle

Abstract

The differentiaton of bone cells is a complex multistep process. Bone is somewhat unusual in that it is very actively and continually remodeled in the adult and that maintenance of its mass in the mature organism is exquisitely sensitive to mechanical as well as chemical signals. Bone is also unique because it consists of a very large amount of extracellular matrix (ECM) that is mineralized. The integrin family of ECM receptors has been shown to play an important role in tissue morphogenesis in several systems. Our studies on the regulation of matrix remodeling enzymes by integrins in rabbit synovial fibroblasts show that two b1 integrin fibronectin (FN) receptor complexes (alpha 5 beta 1 and alpha 4 beta 1) cooperate in detecting subtle changes in the composition of the ECM. As a result of signal transduction by these integrins, the levels of mRNA and protein for several members of the metalloproteinase family are regulated in these cells. We have also used antibody and RGD peptide perturbation studies to determine the significance of cell/ECM interactions to normal osteogenesis. We found that interactions between the cell binding domain of FN and integrins are required for both normal morphogenesis and gene expression in cultured osteoblasts that differentiate to form bone-like tissue in culture. These data lead us to propose that beta 1 integrins play an important role in osteoblast differentiation as well as in bone remodeling.

Original languageEnglish (US)
Pages (from-to)19-28
Number of pages10
JournalASGSB bulletin : publication of the American Society for Gravitational and Space Biology
Volume8
Issue number2
StatePublished - Oct 1995

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Osteoblasts
Integrins
Connective Tissue
Extracellular Matrix
Bone and Bones
Morphogenesis
Integrin alpha5beta1
Integrin beta Chains
Bone Remodeling
Metalloproteases
Fibronectins
Osteogenesis
Cell Communication
Signal Transduction
Fibroblasts
Maintenance
Rabbits
Gene Expression
Messenger RNA
Antibodies

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Integrin-extracellular matrix interactions in connective tissue remodeling and osteoblast differentiation. / Globus, R. K.; Moursi, Amr; Zimmerman, D.; Lull, J.; Damsky, C.

In: ASGSB bulletin : publication of the American Society for Gravitational and Space Biology, Vol. 8, No. 2, 10.1995, p. 19-28.

Research output: Contribution to journalArticle

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