Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots

Elena A. Vidal, Tomás C. Moyano, Gabriel Krouk, Manpreet S. Katari, Milos Tanurdzic, W. R. McCombie, Gloria Coruzzi, Rodrigo A. Gutiérrez

Research output: Contribution to journalArticle

Abstract

Background: Nitrate and other nitrogen metabolites can act as signals that regulate global gene expression in plants. Adaptive changes in plant morphology and physiology triggered by changes in nitrate availability are partly explained by these changes in gene expression. Despite several genome-wide efforts to identify nitrate-regulated genes, no comprehensive study of the Arabidopsis root transcriptome under contrasting nitrate conditions has been carried out.Results: In this work, we employed the Illumina high throughput sequencing technology to perform an integrated analysis of the poly-A + enriched and the small RNA fractions of the Arabidopsis thaliana root transcriptome in response to nitrate treatments. Our sequencing strategy identified new nitrate-regulated genes including 40 genes not represented in the ATH1 Affymetrix GeneChip, a novel nitrate-responsive antisense transcript and a new nitrate responsive miRNA/TARGET module consisting of a novel microRNA, miR5640 and its target, AtPPC3.Conclusions: Sequencing of small RNAs and mRNAs uncovered new genes, and enabled us to develop new hypotheses for nitrate regulation and coordination of carbon and nitrogen metabolism.

Original languageEnglish (US)
Article number701
JournalBMC Genomics
Volume14
Issue number1
DOIs
StatePublished - Oct 11 2013

Fingerprint

Arabidopsis
Nitrates
RNA
Genes
MicroRNAs
Transcriptome
Nitrogen
Plant Physiological Phenomena
RNA Sequence Analysis
Gene Expression
Poly A
Carbon
Genome
Technology
Messenger RNA

Keywords

  • Arabidopsis
  • MicroRNA
  • Nitrate
  • RNA-seq
  • Roots
  • Transcriptomics

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Medicine(all)

Cite this

Vidal, E. A., Moyano, T. C., Krouk, G., Katari, M. S., Tanurdzic, M., McCombie, W. R., ... Gutiérrez, R. A. (2013). Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots. BMC Genomics, 14(1), [701]. https://doi.org/10.1186/1471-2164-14-701

Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots. / Vidal, Elena A.; Moyano, Tomás C.; Krouk, Gabriel; Katari, Manpreet S.; Tanurdzic, Milos; McCombie, W. R.; Coruzzi, Gloria; Gutiérrez, Rodrigo A.

In: BMC Genomics, Vol. 14, No. 1, 701, 11.10.2013.

Research output: Contribution to journalArticle

Vidal, EA, Moyano, TC, Krouk, G, Katari, MS, Tanurdzic, M, McCombie, WR, Coruzzi, G & Gutiérrez, RA 2013, 'Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots', BMC Genomics, vol. 14, no. 1, 701. https://doi.org/10.1186/1471-2164-14-701
Vidal, Elena A. ; Moyano, Tomás C. ; Krouk, Gabriel ; Katari, Manpreet S. ; Tanurdzic, Milos ; McCombie, W. R. ; Coruzzi, Gloria ; Gutiérrez, Rodrigo A. / Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots. In: BMC Genomics. 2013 ; Vol. 14, No. 1.
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