Integrated microfluidic probe station

C. M. Perrault, Mohammad Qasaimeh, T. Brastaviceanu, K. Anderson, Y. Kabakibo, D. Juncker

    Research output: Contribution to journalArticle

    Abstract

    The microfluidic probe (MFP) consists of a flat, blunt tip with two apertures for the injection and reaspiration of a microjet into a solution-thus hydrodynamically confining the microjet-and is operated atop an inverted microscope that enables live imaging. By scanning across a surface, the microjet can be used for surface processing with the capability of both depositing and removing material; as it operates under immersed conditions, sensitive biological materials and living cells can be processed. During scanning, the MFP is kept immobile and centered over the objective of the inverted microscope, a few micrometers above a substrate that is displaced by moving the microscope stage and that is flushed continuously with the microjet. For consistent and reproducible surface processing, the gap between the MFP and the substrate, the MFP's alignment, the scanning speed, the injection and aspiration flow rates, and the image capture need all to be controlled and synchronized. Here, we present an automated MFP station that integrates all of these functionalities and automates the key operational parameters. A custom software program is used to control an independent motorized Z stage for adjusting the gap, a motorized microscope stage for scanning the substrate, up to 16 syringe pumps for injecting and aspirating fluids, and an inverted fluorescence microscope equipped with a charge-coupled device camera. The parallelism between the MFP and the substrate is adjusted using manual goniometer at the beginning of the experiment. The alignment of the injection and aspiration apertures along the scanning axis is performed using a newly designed MFP screw holder. We illustrate the integrated MFP station by the programmed, automated patterning of fluorescently labeled biotin on a streptavidin-coated surface.

    Original languageEnglish (US)
    Article number115107
    JournalReview of Scientific Instruments
    Volume81
    Issue number11
    DOIs
    StatePublished - Nov 1 2010

    Fingerprint

    Microfluidics
    stations
    probes
    Microscopes
    microscopes
    Scanning
    scanning
    Substrates
    injection
    apertures
    alignment
    Goniometers
    syringes
    Syringes
    goniometers
    vacuum
    biotin
    screws
    CCD cameras
    holders

    ASJC Scopus subject areas

    • Instrumentation

    Cite this

    Perrault, C. M., Qasaimeh, M., Brastaviceanu, T., Anderson, K., Kabakibo, Y., & Juncker, D. (2010). Integrated microfluidic probe station. Review of Scientific Instruments, 81(11), [115107]. https://doi.org/10.1063/1.3497302

    Integrated microfluidic probe station. / Perrault, C. M.; Qasaimeh, Mohammad; Brastaviceanu, T.; Anderson, K.; Kabakibo, Y.; Juncker, D.

    In: Review of Scientific Instruments, Vol. 81, No. 11, 115107, 01.11.2010.

    Research output: Contribution to journalArticle

    Perrault, CM, Qasaimeh, M, Brastaviceanu, T, Anderson, K, Kabakibo, Y & Juncker, D 2010, 'Integrated microfluidic probe station', Review of Scientific Instruments, vol. 81, no. 11, 115107. https://doi.org/10.1063/1.3497302
    Perrault CM, Qasaimeh M, Brastaviceanu T, Anderson K, Kabakibo Y, Juncker D. Integrated microfluidic probe station. Review of Scientific Instruments. 2010 Nov 1;81(11). 115107. https://doi.org/10.1063/1.3497302
    Perrault, C. M. ; Qasaimeh, Mohammad ; Brastaviceanu, T. ; Anderson, K. ; Kabakibo, Y. ; Juncker, D. / Integrated microfluidic probe station. In: Review of Scientific Instruments. 2010 ; Vol. 81, No. 11.
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