Insights into Mollusk Shell Formation

Interlamellar and Lamellar-Specific Nacre Protein Hydrogels Differ in Ion Interaction Signatures

Martin Pendola, John Evans

Research output: Contribution to journalArticle

Abstract

In the mollusk shell nacre layer, there exist hydrogelator proteomes that play important roles in the formation of the mineral phase. Two of these proteomes, the intracrystalline and the framework, reside in the interior and exterior, respectively, of the nacre tablets. To date there is no clear evidence of what distinguishes an intracrystalline protein from a framework protein regarding the nucleation process. Using Eu(III), phosphate anions, and recombinant versions of the intracrystalline protein, AP7 and the framework protein, n16.3 we probed each protein hydrogel for its interactions with these model ions. Fluorescence spectroscopy of Eu(III) interactions with both protein hydrogels revealed that r-AP7 exhibited enhanced effects on Eu(III) fluorescence compared to r-n16.3, and, 31P NMR experiments demonstrated that r-AP7 had a more significant impact on phosphate anions compared to r-n16.3. Thus, r-AP7 was found to be more of an ion "disruptor" than r-n16.3. Interestingly, these findings correlate with the particle size distributions and internal structure of the hydrogel particles themselves, suggesting that the physical and chemical properties of the hydrogels dictate hydrogel-ion interactions. In conclusion, we confirm that hydrogelator proteomes possess distinguishable ion interaction properties that may impact the nucleation processes in these regions and control the overall formation of mesoscale nacre tablets.

Original languageEnglish (US)
Pages (from-to)1161-1168
Number of pages8
JournalJournal of Physical Chemistry B
Volume122
Issue number3
DOIs
StatePublished - Jan 25 2018

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Nacre
mollusks
Hydrogels
proteome
signatures
Ions
proteins
Proteins
Hydrogel
Proteome
ions
tablets
interactions
Tablets
Anions
phosphates
Nucleation
Phosphates
nucleation
anions

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

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abstract = "In the mollusk shell nacre layer, there exist hydrogelator proteomes that play important roles in the formation of the mineral phase. Two of these proteomes, the intracrystalline and the framework, reside in the interior and exterior, respectively, of the nacre tablets. To date there is no clear evidence of what distinguishes an intracrystalline protein from a framework protein regarding the nucleation process. Using Eu(III), phosphate anions, and recombinant versions of the intracrystalline protein, AP7 and the framework protein, n16.3 we probed each protein hydrogel for its interactions with these model ions. Fluorescence spectroscopy of Eu(III) interactions with both protein hydrogels revealed that r-AP7 exhibited enhanced effects on Eu(III) fluorescence compared to r-n16.3, and, 31P NMR experiments demonstrated that r-AP7 had a more significant impact on phosphate anions compared to r-n16.3. Thus, r-AP7 was found to be more of an ion {"}disruptor{"} than r-n16.3. Interestingly, these findings correlate with the particle size distributions and internal structure of the hydrogel particles themselves, suggesting that the physical and chemical properties of the hydrogels dictate hydrogel-ion interactions. In conclusion, we confirm that hydrogelator proteomes possess distinguishable ion interaction properties that may impact the nucleation processes in these regions and control the overall formation of mesoscale nacre tablets.",
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