Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ

Deepak Saxena, G. Stotzky

Research output: Contribution to journalArticle

Abstract

The insecticidal toxin encoded by the cry1Ab gene from Bacillus thuringiensis was released in root exudates from transgenic Bt corn during 40 days of growth in soil amended to 0, 3, 6, 9, or 12% (v/v) with montmorillonite or kaolinite in a plant growth room and from plants grown to maturity in the field. The presence of the toxin in rhizosphere soil was determined by immunological and larvicidal assays. No toxin was detected in any soils from isogenic non-Bt corn or without plants. Persistence of the toxin was apparently the result of its binding on surface-active particles in the soils, which reduced the biodegradation of the toxin. The release of the toxin could enhance the control of insect pests or constitute a hazard to nontarget organisms, including the microbiota of soil, and increase the selection of toxin-resistant target insects. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)35-39
Number of pages5
JournalFEMS Microbiology Ecology
Volume33
Issue number1
DOIs
StatePublished - Jul 2000

Fingerprint

Bacillus thuringiensis
toxin
Zea mays
Soil
maize
soil
Insect Control
Bentonite
Kaolin
Pest Control
Rhizosphere
Microbiota
Exudates and Transudates
Growth
insect
nontarget organism
Insects
In Vitro Techniques
in situ
montmorillonite

Keywords

  • Bacillus thuringiensis
  • Clay mineral
  • European corn borer
  • Insecticidal toxin
  • Root exudate
  • Transgenic Bt corn

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ. / Saxena, Deepak; Stotzky, G.

In: FEMS Microbiology Ecology, Vol. 33, No. 1, 07.2000, p. 35-39.

Research output: Contribution to journalArticle

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