Inhibitory effects of parathyroid hormone on growth of osteogenic sarcoma cells

Nicola Partridge, Anne Louise Opie, Rebecca T. Opie, T. John Martin

Research output: Contribution to journalArticle

Abstract

The effects of the bone resorbing hormone, parathyroid hormone (PTH), on the growth of malignant osteoblastic cells have been examined. The malignant osteoblastic cells were a clonal line (UMR 106) derived from a transplantable rat osteogenic sarcoma. The predominant effect of PTH at doses above 10-10 M was an inhibition of replication and DNA synthesis. Replication was decreased by PTH in both the presence or absence of serum and at various cell seeding densities. Both bovine PTH (1-84) and the synthetic hormone, human PTH (1-34), inhibited replication, but with bovine hormone being an order of magnitude more potent. The effects could be observed in as short a time as 6 hours with DNA synthesis and 24 hours with replication.

Original languageEnglish (US)
Pages (from-to)519-525
Number of pages7
JournalCalcified Tissue International
Volume37
Issue number5
DOIs
StatePublished - Sep 1985

Fingerprint

Osteosarcoma
Parathyroid Hormone
Hormones
Growth
Teriparatide
DNA Replication
Cell Count
Bone and Bones
DNA
Serum

Keywords

  • DNA synthesis
  • Osteoblast
  • Parathyroid hormone
  • Replication

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Inhibitory effects of parathyroid hormone on growth of osteogenic sarcoma cells. / Partridge, Nicola; Louise Opie, Anne; Opie, Rebecca T.; John Martin, T.

In: Calcified Tissue International, Vol. 37, No. 5, 09.1985, p. 519-525.

Research output: Contribution to journalArticle

Partridge, Nicola ; Louise Opie, Anne ; Opie, Rebecca T. ; John Martin, T. / Inhibitory effects of parathyroid hormone on growth of osteogenic sarcoma cells. In: Calcified Tissue International. 1985 ; Vol. 37, No. 5. pp. 519-525.
@article{9d8c9160488a41b2a36c9c5342990d65,
title = "Inhibitory effects of parathyroid hormone on growth of osteogenic sarcoma cells",
abstract = "The effects of the bone resorbing hormone, parathyroid hormone (PTH), on the growth of malignant osteoblastic cells have been examined. The malignant osteoblastic cells were a clonal line (UMR 106) derived from a transplantable rat osteogenic sarcoma. The predominant effect of PTH at doses above 10-10 M was an inhibition of replication and DNA synthesis. Replication was decreased by PTH in both the presence or absence of serum and at various cell seeding densities. Both bovine PTH (1-84) and the synthetic hormone, human PTH (1-34), inhibited replication, but with bovine hormone being an order of magnitude more potent. The effects could be observed in as short a time as 6 hours with DNA synthesis and 24 hours with replication.",
keywords = "DNA synthesis, Osteoblast, Parathyroid hormone, Replication",
author = "Nicola Partridge and {Louise Opie}, Anne and Opie, {Rebecca T.} and {John Martin}, T.",
year = "1985",
month = "9",
doi = "10.1007/BF02557835",
language = "English (US)",
volume = "37",
pages = "519--525",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Inhibitory effects of parathyroid hormone on growth of osteogenic sarcoma cells

AU - Partridge, Nicola

AU - Louise Opie, Anne

AU - Opie, Rebecca T.

AU - John Martin, T.

PY - 1985/9

Y1 - 1985/9

N2 - The effects of the bone resorbing hormone, parathyroid hormone (PTH), on the growth of malignant osteoblastic cells have been examined. The malignant osteoblastic cells were a clonal line (UMR 106) derived from a transplantable rat osteogenic sarcoma. The predominant effect of PTH at doses above 10-10 M was an inhibition of replication and DNA synthesis. Replication was decreased by PTH in both the presence or absence of serum and at various cell seeding densities. Both bovine PTH (1-84) and the synthetic hormone, human PTH (1-34), inhibited replication, but with bovine hormone being an order of magnitude more potent. The effects could be observed in as short a time as 6 hours with DNA synthesis and 24 hours with replication.

AB - The effects of the bone resorbing hormone, parathyroid hormone (PTH), on the growth of malignant osteoblastic cells have been examined. The malignant osteoblastic cells were a clonal line (UMR 106) derived from a transplantable rat osteogenic sarcoma. The predominant effect of PTH at doses above 10-10 M was an inhibition of replication and DNA synthesis. Replication was decreased by PTH in both the presence or absence of serum and at various cell seeding densities. Both bovine PTH (1-84) and the synthetic hormone, human PTH (1-34), inhibited replication, but with bovine hormone being an order of magnitude more potent. The effects could be observed in as short a time as 6 hours with DNA synthesis and 24 hours with replication.

KW - DNA synthesis

KW - Osteoblast

KW - Parathyroid hormone

KW - Replication

UR - http://www.scopus.com/inward/record.url?scp=0022256670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022256670&partnerID=8YFLogxK

U2 - 10.1007/BF02557835

DO - 10.1007/BF02557835

M3 - Article

C2 - 3933791

AN - SCOPUS:0022256670

VL - 37

SP - 519

EP - 525

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 5

ER -