Ingested particles reduce susceptibility of insect larvae to Bacillus thuringiensis

Eitan Ben-Dov, Deepak Saxena, Q. Wang, R. Manasherob, S. Boussiba, A. Zaritsky

Research output: Contribution to journalArticle

Abstract

Susceptibility to Bacillus thuringiensis of mosquito and lepidopteran larvae is affected by feeding behaviour and nutritional value of the available food. Reduced mortality is attributed to feeding inhibition and dilution of the pathogen in the presence of nutritional and inert particles, which limit the amount of ingested toxin. These reasons are, however, not sufficient to explain the data presented here. Values of LC50 (the concentration that kills 50% of exposed population) of B. thuringiensis subsp. israelensis (Berliner) against Aedes aegypti (L.) larvae and of B. thuringiensis subsp. kenyae (Berliner) against Spodoptera littoralis (Boisduval) larvae were about 20-217 and 2.3-44-fold higher, respectively, in the presence of nutritional or biologically inert (non-nutritional) particles than without. The number of B. thuringiensis spores in carcasses of B. thuringiensis -killed A. aegypti and S. littoralis larvae were between 1.9 and 5.6-fold and between 8.5 and 12-fold higher, respectively, in the presence of particles than without. In all cases, non-nutritional particles better protected the exposed larvae than nutritious particles. We propose that another basic mechanism exists, that ingested particles protect midgut epithelial cells by covering their surface and thus preventing availability of the toxin to the gut receptors. Understanding the defence mechanisms of insects against B. thuringiensis toxicity may lead to improved pest management methods.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalJournal of Applied Entomology
Volume127
Issue number3
DOIs
StatePublished - Apr 2003

Fingerprint

Bacillus thuringiensis
insect larvae
larvae
Spodoptera littoralis
Aedes aegypti
Bacillus thuringiensis subsp. kenyae
toxins
Bacillus thuringiensis subsp. israelensis
at-risk population
pest management
defense mechanisms
midgut
lethal concentration 50
feeding behavior
Culicidae
epithelial cells
digestive system
spores
nutritive value
Lepidoptera

ASJC Scopus subject areas

  • Insect Science

Cite this

Ingested particles reduce susceptibility of insect larvae to Bacillus thuringiensis. / Ben-Dov, Eitan; Saxena, Deepak; Wang, Q.; Manasherob, R.; Boussiba, S.; Zaritsky, A.

In: Journal of Applied Entomology, Vol. 127, No. 3, 04.2003, p. 146-152.

Research output: Contribution to journalArticle

Ben-Dov, Eitan ; Saxena, Deepak ; Wang, Q. ; Manasherob, R. ; Boussiba, S. ; Zaritsky, A. / Ingested particles reduce susceptibility of insect larvae to Bacillus thuringiensis. In: Journal of Applied Entomology. 2003 ; Vol. 127, No. 3. pp. 146-152.
@article{ab32117ea006401aa6968c933ae17f69,
title = "Ingested particles reduce susceptibility of insect larvae to Bacillus thuringiensis",
abstract = "Susceptibility to Bacillus thuringiensis of mosquito and lepidopteran larvae is affected by feeding behaviour and nutritional value of the available food. Reduced mortality is attributed to feeding inhibition and dilution of the pathogen in the presence of nutritional and inert particles, which limit the amount of ingested toxin. These reasons are, however, not sufficient to explain the data presented here. Values of LC50 (the concentration that kills 50{\%} of exposed population) of B. thuringiensis subsp. israelensis (Berliner) against Aedes aegypti (L.) larvae and of B. thuringiensis subsp. kenyae (Berliner) against Spodoptera littoralis (Boisduval) larvae were about 20-217 and 2.3-44-fold higher, respectively, in the presence of nutritional or biologically inert (non-nutritional) particles than without. The number of B. thuringiensis spores in carcasses of B. thuringiensis -killed A. aegypti and S. littoralis larvae were between 1.9 and 5.6-fold and between 8.5 and 12-fold higher, respectively, in the presence of particles than without. In all cases, non-nutritional particles better protected the exposed larvae than nutritious particles. We propose that another basic mechanism exists, that ingested particles protect midgut epithelial cells by covering their surface and thus preventing availability of the toxin to the gut receptors. Understanding the defence mechanisms of insects against B. thuringiensis toxicity may lead to improved pest management methods.",
author = "Eitan Ben-Dov and Deepak Saxena and Q. Wang and R. Manasherob and S. Boussiba and A. Zaritsky",
year = "2003",
month = "4",
doi = "10.1046/j.1439-0418.2003.00732.x",
language = "English (US)",
volume = "127",
pages = "146--152",
journal = "Journal of Applied Entomology",
issn = "0931-2048",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Ingested particles reduce susceptibility of insect larvae to Bacillus thuringiensis

AU - Ben-Dov, Eitan

AU - Saxena, Deepak

AU - Wang, Q.

AU - Manasherob, R.

AU - Boussiba, S.

AU - Zaritsky, A.

PY - 2003/4

Y1 - 2003/4

N2 - Susceptibility to Bacillus thuringiensis of mosquito and lepidopteran larvae is affected by feeding behaviour and nutritional value of the available food. Reduced mortality is attributed to feeding inhibition and dilution of the pathogen in the presence of nutritional and inert particles, which limit the amount of ingested toxin. These reasons are, however, not sufficient to explain the data presented here. Values of LC50 (the concentration that kills 50% of exposed population) of B. thuringiensis subsp. israelensis (Berliner) against Aedes aegypti (L.) larvae and of B. thuringiensis subsp. kenyae (Berliner) against Spodoptera littoralis (Boisduval) larvae were about 20-217 and 2.3-44-fold higher, respectively, in the presence of nutritional or biologically inert (non-nutritional) particles than without. The number of B. thuringiensis spores in carcasses of B. thuringiensis -killed A. aegypti and S. littoralis larvae were between 1.9 and 5.6-fold and between 8.5 and 12-fold higher, respectively, in the presence of particles than without. In all cases, non-nutritional particles better protected the exposed larvae than nutritious particles. We propose that another basic mechanism exists, that ingested particles protect midgut epithelial cells by covering their surface and thus preventing availability of the toxin to the gut receptors. Understanding the defence mechanisms of insects against B. thuringiensis toxicity may lead to improved pest management methods.

AB - Susceptibility to Bacillus thuringiensis of mosquito and lepidopteran larvae is affected by feeding behaviour and nutritional value of the available food. Reduced mortality is attributed to feeding inhibition and dilution of the pathogen in the presence of nutritional and inert particles, which limit the amount of ingested toxin. These reasons are, however, not sufficient to explain the data presented here. Values of LC50 (the concentration that kills 50% of exposed population) of B. thuringiensis subsp. israelensis (Berliner) against Aedes aegypti (L.) larvae and of B. thuringiensis subsp. kenyae (Berliner) against Spodoptera littoralis (Boisduval) larvae were about 20-217 and 2.3-44-fold higher, respectively, in the presence of nutritional or biologically inert (non-nutritional) particles than without. The number of B. thuringiensis spores in carcasses of B. thuringiensis -killed A. aegypti and S. littoralis larvae were between 1.9 and 5.6-fold and between 8.5 and 12-fold higher, respectively, in the presence of particles than without. In all cases, non-nutritional particles better protected the exposed larvae than nutritious particles. We propose that another basic mechanism exists, that ingested particles protect midgut epithelial cells by covering their surface and thus preventing availability of the toxin to the gut receptors. Understanding the defence mechanisms of insects against B. thuringiensis toxicity may lead to improved pest management methods.

UR - http://www.scopus.com/inward/record.url?scp=0037398442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037398442&partnerID=8YFLogxK

U2 - 10.1046/j.1439-0418.2003.00732.x

DO - 10.1046/j.1439-0418.2003.00732.x

M3 - Article

VL - 127

SP - 146

EP - 152

JO - Journal of Applied Entomology

JF - Journal of Applied Entomology

SN - 0931-2048

IS - 3

ER -