Influence of surface charge, binding site residues and glycosylation on Thielavia terrestris cutinase biochemical characteristics

Abhijit N. Shirke, Danielle Basore, Samantha Holton, An Su, Evan Baugh, Glenn Butterfoss, George Makhatadze, Christopher Bystroff, Richard A. Gross

Research output: Contribution to journalArticle

Abstract

Cutinases are esterases of industrial importance for applications in recycling and surface modification of polyesters. The cutinase from Thielavia terrestris (TtC) is distinct in terms of its ability to retain its stability and activity in acidic pH. Stability and activity in acidic pHs are desirable for esterases as the pH of the reaction tends to go down with the generation of acid. The pH stability and activity are governed by the charged state of the residues involved in catalysis or in substrate binding. In this study, we performed the detailed structural and biochemical characterization of TtC coupled with surface charge analysis to understand its acidic tolerance. The stability of TtC in acidic pH was rationalized by evaluating the contribution of charge interactions to the Gibbs free energy of unfolding at varying pHs. The activity of TtC was found to be limited by substrate binding affinity, which is a function of the surface charge. Additionally, the presence of glycosylation affects the biochemical characteristics of TtC owing to steric interactions with residues involved in substrate binding.

Original languageEnglish (US)
Pages (from-to)4435-4446
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume100
Issue number10
DOIs
StatePublished - May 1 2016

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Glycosylation
Binding Sites
Esterases
Polyesters
Recycling
Catalysis
Acids
cutinase

Keywords

  • Biochemical characterization
  • Cutinase
  • Glycosylation
  • Polyester hydrolysis
  • Substrate binding
  • Surface charge

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Influence of surface charge, binding site residues and glycosylation on Thielavia terrestris cutinase biochemical characteristics. / Shirke, Abhijit N.; Basore, Danielle; Holton, Samantha; Su, An; Baugh, Evan; Butterfoss, Glenn; Makhatadze, George; Bystroff, Christopher; Gross, Richard A.

In: Applied Microbiology and Biotechnology, Vol. 100, No. 10, 01.05.2016, p. 4435-4446.

Research output: Contribution to journalArticle

Shirke, Abhijit N. ; Basore, Danielle ; Holton, Samantha ; Su, An ; Baugh, Evan ; Butterfoss, Glenn ; Makhatadze, George ; Bystroff, Christopher ; Gross, Richard A. / Influence of surface charge, binding site residues and glycosylation on Thielavia terrestris cutinase biochemical characteristics. In: Applied Microbiology and Biotechnology. 2016 ; Vol. 100, No. 10. pp. 4435-4446.
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AU - Baugh, Evan

AU - Butterfoss, Glenn

AU - Makhatadze, George

AU - Bystroff, Christopher

AU - Gross, Richard A.

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