Influence of interleukin 10 polymorphisms -592 and -1082 to the HIV, HBV and HCV serostatus among intravenous drug users

Eveli Kallas, Kristi Huik, Merit Pauskar, Ene Ly Jõgeda, Tõnis Karki, Don Des Jarlais, Anneli Uusküla, Radko Avi, Irja Lutsar

Research output: Contribution to journalArticle

Abstract

Background: Interleukin 10 (IL-10) is a multifunctional cytokine produced by macrophages, monocytes, and T-helper cells. Two polymorphisms at positions -592 and -1082 have been associated with HIV susceptibility. However, their associations with susceptibility to HIV and its co-infections among intravenous drug users (IDUs) are largely unknown. Methods: A total of 345 IDUs were recruited. Of the 173 HIV negative IDUs, 20 were classified as highly exposed HIV seronegative subjects (HESNs). A control group consisted of 496 blood donors; all HIV, HCV, and HBV negative. The IL-10-592C/A and -1082A/G were determined using TaqMan allelic discrimination assay. Results: Of the IDUs, 50% were HIV positive, 89% HCV positive, 67% HBV positive and 41% had triple infection. IL-10-592C allele and -1082A allele were the most common and the -1082AG/-592CC was the most common genotype pair. All HESNs exhibited -1082A allele as compared to 81.4% of the HIV positive IDUs and 79% of donors (p= 0.029 and p= 0.019, respectively). None of HESNs had GG/CC genotype pair compared with 18.6% of HIV positive IDUs and 21.0% of donors (p= 0.029 and p= 0.019, respectively). The possession of -592AC and genotype pair AG/AC were associated with the decreased odds of HBV infection (OR. = 0.28; 95% CI 0.09-0.87; p= 0.028 and OR. = 0.19; 95% CI 0.06-0.61; p= 0.052, respectively). Conclusions: The presence of low producing IL-10-1082A and -592A alleles and their containing genetic variants protect highly exposed IDUs against acquisition of HIV and HBV infections.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalInfection, Genetics and Evolution
Volume30
DOIs
StatePublished - Mar 1 2015

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drug user
human immunodeficiency virus
interleukin-10
Drug Users
Interleukin-10
polymorphism
HIV
genetic polymorphism
drugs
alleles
Alleles
allele
Genotype
genotype
infection
Tissue Donors
mixed infection
monocytes
Helper-Inducer T-Lymphocytes
Infection

Keywords

  • Co-infection
  • Genetic factors
  • HIV susceptibility
  • IDU
  • IL-10
  • Inflammation

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Influence of interleukin 10 polymorphisms -592 and -1082 to the HIV, HBV and HCV serostatus among intravenous drug users. / Kallas, Eveli; Huik, Kristi; Pauskar, Merit; Jõgeda, Ene Ly; Karki, Tõnis; Des Jarlais, Don; Uusküla, Anneli; Avi, Radko; Lutsar, Irja.

In: Infection, Genetics and Evolution, Vol. 30, 01.03.2015, p. 175-180.

Research output: Contribution to journalArticle

Kallas, Eveli ; Huik, Kristi ; Pauskar, Merit ; Jõgeda, Ene Ly ; Karki, Tõnis ; Des Jarlais, Don ; Uusküla, Anneli ; Avi, Radko ; Lutsar, Irja. / Influence of interleukin 10 polymorphisms -592 and -1082 to the HIV, HBV and HCV serostatus among intravenous drug users. In: Infection, Genetics and Evolution. 2015 ; Vol. 30. pp. 175-180.
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title = "Influence of interleukin 10 polymorphisms -592 and -1082 to the HIV, HBV and HCV serostatus among intravenous drug users",
abstract = "Background: Interleukin 10 (IL-10) is a multifunctional cytokine produced by macrophages, monocytes, and T-helper cells. Two polymorphisms at positions -592 and -1082 have been associated with HIV susceptibility. However, their associations with susceptibility to HIV and its co-infections among intravenous drug users (IDUs) are largely unknown. Methods: A total of 345 IDUs were recruited. Of the 173 HIV negative IDUs, 20 were classified as highly exposed HIV seronegative subjects (HESNs). A control group consisted of 496 blood donors; all HIV, HCV, and HBV negative. The IL-10-592C/A and -1082A/G were determined using TaqMan allelic discrimination assay. Results: Of the IDUs, 50{\%} were HIV positive, 89{\%} HCV positive, 67{\%} HBV positive and 41{\%} had triple infection. IL-10-592C allele and -1082A allele were the most common and the -1082AG/-592CC was the most common genotype pair. All HESNs exhibited -1082A allele as compared to 81.4{\%} of the HIV positive IDUs and 79{\%} of donors (p= 0.029 and p= 0.019, respectively). None of HESNs had GG/CC genotype pair compared with 18.6{\%} of HIV positive IDUs and 21.0{\%} of donors (p= 0.029 and p= 0.019, respectively). The possession of -592AC and genotype pair AG/AC were associated with the decreased odds of HBV infection (OR. = 0.28; 95{\%} CI 0.09-0.87; p= 0.028 and OR. = 0.19; 95{\%} CI 0.06-0.61; p= 0.052, respectively). Conclusions: The presence of low producing IL-10-1082A and -592A alleles and their containing genetic variants protect highly exposed IDUs against acquisition of HIV and HBV infections.",
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T1 - Influence of interleukin 10 polymorphisms -592 and -1082 to the HIV, HBV and HCV serostatus among intravenous drug users

AU - Kallas, Eveli

AU - Huik, Kristi

AU - Pauskar, Merit

AU - Jõgeda, Ene Ly

AU - Karki, Tõnis

AU - Des Jarlais, Don

AU - Uusküla, Anneli

AU - Avi, Radko

AU - Lutsar, Irja

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Background: Interleukin 10 (IL-10) is a multifunctional cytokine produced by macrophages, monocytes, and T-helper cells. Two polymorphisms at positions -592 and -1082 have been associated with HIV susceptibility. However, their associations with susceptibility to HIV and its co-infections among intravenous drug users (IDUs) are largely unknown. Methods: A total of 345 IDUs were recruited. Of the 173 HIV negative IDUs, 20 were classified as highly exposed HIV seronegative subjects (HESNs). A control group consisted of 496 blood donors; all HIV, HCV, and HBV negative. The IL-10-592C/A and -1082A/G were determined using TaqMan allelic discrimination assay. Results: Of the IDUs, 50% were HIV positive, 89% HCV positive, 67% HBV positive and 41% had triple infection. IL-10-592C allele and -1082A allele were the most common and the -1082AG/-592CC was the most common genotype pair. All HESNs exhibited -1082A allele as compared to 81.4% of the HIV positive IDUs and 79% of donors (p= 0.029 and p= 0.019, respectively). None of HESNs had GG/CC genotype pair compared with 18.6% of HIV positive IDUs and 21.0% of donors (p= 0.029 and p= 0.019, respectively). The possession of -592AC and genotype pair AG/AC were associated with the decreased odds of HBV infection (OR. = 0.28; 95% CI 0.09-0.87; p= 0.028 and OR. = 0.19; 95% CI 0.06-0.61; p= 0.052, respectively). Conclusions: The presence of low producing IL-10-1082A and -592A alleles and their containing genetic variants protect highly exposed IDUs against acquisition of HIV and HBV infections.

AB - Background: Interleukin 10 (IL-10) is a multifunctional cytokine produced by macrophages, monocytes, and T-helper cells. Two polymorphisms at positions -592 and -1082 have been associated with HIV susceptibility. However, their associations with susceptibility to HIV and its co-infections among intravenous drug users (IDUs) are largely unknown. Methods: A total of 345 IDUs were recruited. Of the 173 HIV negative IDUs, 20 were classified as highly exposed HIV seronegative subjects (HESNs). A control group consisted of 496 blood donors; all HIV, HCV, and HBV negative. The IL-10-592C/A and -1082A/G were determined using TaqMan allelic discrimination assay. Results: Of the IDUs, 50% were HIV positive, 89% HCV positive, 67% HBV positive and 41% had triple infection. IL-10-592C allele and -1082A allele were the most common and the -1082AG/-592CC was the most common genotype pair. All HESNs exhibited -1082A allele as compared to 81.4% of the HIV positive IDUs and 79% of donors (p= 0.029 and p= 0.019, respectively). None of HESNs had GG/CC genotype pair compared with 18.6% of HIV positive IDUs and 21.0% of donors (p= 0.029 and p= 0.019, respectively). The possession of -592AC and genotype pair AG/AC were associated with the decreased odds of HBV infection (OR. = 0.28; 95% CI 0.09-0.87; p= 0.028 and OR. = 0.19; 95% CI 0.06-0.61; p= 0.052, respectively). Conclusions: The presence of low producing IL-10-1082A and -592A alleles and their containing genetic variants protect highly exposed IDUs against acquisition of HIV and HBV infections.

KW - Co-infection

KW - Genetic factors

KW - HIV susceptibility

KW - IDU

KW - IL-10

KW - Inflammation

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