Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing

Louis Terracio, L. Ronnstrand, A. Tingstrom, K. Rubin, L. Claesson-Welsh, K. Funa, C. H. Heldin

Research output: Contribution to journalArticle

Abstract

The expression of platelet-derived growth factor (PDGF) receptors in porcine uterus and human skin in situ, was compared with that of cultured primary cells isolated from the same tissues, PDGF receptor expression was examined by monoclonal antibodies specific for the B type PDGF receptor and by RNA/RNA in situ hybridization with a probe constructed from a cDNA clone encoding the B type PDGF receptor. In porcine uterus tissue both mRNA and the protein product for the PDGF receptor were detected in the endometrium; the myometrium, in contrast, contained much lower amounts. Moreover, freshly isolated myometrial cells were devoid of PDGF receptors. However, after 1 d in culture receptors appeared, and after 2 wk of culturing essentially all of the myometrial cells stained positively with the anti-PDGF receptor antibodies and contained PDGF receptor mRNA. Similarly, B type PDGF receptors were not detected in normal human skin, but fibroblast-like cells from explant cultures of human skin possessed PDGF receptors. When determined by immunoblotting, porcine uterus myometrial membranes contained ~20% of the PDGF receptor antigen compared with the amount found in endometrial membranes. In addition, PDGF stimulated the phosphorylation of a 175-kD component, most likely representing autophosphorylation of the B type of PDGF receptor in endometrial membranes, whereas only a marginal phosphorylation was seen in myometrial membranes. Taken together, these results demonstrate that PDGF receptor expression varies in normal tissues and that fibroblasts and smooth muscle cells do not uniformly express the receptor in situ. Furthermore, fibroblasts and smooth muscle cells that are released from tissues are induced to express PDGF receptors in response to cell culturing. The data suggest that, in addition to the availability of the ligand, PDGF-mediated cell growth in vivo is dependent on factors regulating expression of the receptor.

Original languageEnglish (US)
Pages (from-to)1947-1957
Number of pages11
JournalJournal of Cell Biology
Volume107
Issue number5
StatePublished - 1988

Fingerprint

Platelet-Derived Growth Factor Receptors
Smooth Muscle Myocytes
Fibroblasts
Uterus
Swine
Membranes
Platelet-Derived Growth Factor
Skin
Phosphorylation
RNA
Messenger RNA
Myometrium
Endometrium
Immunoblotting

ASJC Scopus subject areas

  • Cell Biology

Cite this

Terracio, L., Ronnstrand, L., Tingstrom, A., Rubin, K., Claesson-Welsh, L., Funa, K., & Heldin, C. H. (1988). Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing. Journal of Cell Biology, 107(5), 1947-1957.

Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing. / Terracio, Louis; Ronnstrand, L.; Tingstrom, A.; Rubin, K.; Claesson-Welsh, L.; Funa, K.; Heldin, C. H.

In: Journal of Cell Biology, Vol. 107, No. 5, 1988, p. 1947-1957.

Research output: Contribution to journalArticle

Terracio, L, Ronnstrand, L, Tingstrom, A, Rubin, K, Claesson-Welsh, L, Funa, K & Heldin, CH 1988, 'Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing', Journal of Cell Biology, vol. 107, no. 5, pp. 1947-1957.
Terracio, Louis ; Ronnstrand, L. ; Tingstrom, A. ; Rubin, K. ; Claesson-Welsh, L. ; Funa, K. ; Heldin, C. H. / Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing. In: Journal of Cell Biology. 1988 ; Vol. 107, No. 5. pp. 1947-1957.
@article{714bc58619994b8eb66ca02afd401eb1,
title = "Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing",
abstract = "The expression of platelet-derived growth factor (PDGF) receptors in porcine uterus and human skin in situ, was compared with that of cultured primary cells isolated from the same tissues, PDGF receptor expression was examined by monoclonal antibodies specific for the B type PDGF receptor and by RNA/RNA in situ hybridization with a probe constructed from a cDNA clone encoding the B type PDGF receptor. In porcine uterus tissue both mRNA and the protein product for the PDGF receptor were detected in the endometrium; the myometrium, in contrast, contained much lower amounts. Moreover, freshly isolated myometrial cells were devoid of PDGF receptors. However, after 1 d in culture receptors appeared, and after 2 wk of culturing essentially all of the myometrial cells stained positively with the anti-PDGF receptor antibodies and contained PDGF receptor mRNA. Similarly, B type PDGF receptors were not detected in normal human skin, but fibroblast-like cells from explant cultures of human skin possessed PDGF receptors. When determined by immunoblotting, porcine uterus myometrial membranes contained ~20{\%} of the PDGF receptor antigen compared with the amount found in endometrial membranes. In addition, PDGF stimulated the phosphorylation of a 175-kD component, most likely representing autophosphorylation of the B type of PDGF receptor in endometrial membranes, whereas only a marginal phosphorylation was seen in myometrial membranes. Taken together, these results demonstrate that PDGF receptor expression varies in normal tissues and that fibroblasts and smooth muscle cells do not uniformly express the receptor in situ. Furthermore, fibroblasts and smooth muscle cells that are released from tissues are induced to express PDGF receptors in response to cell culturing. The data suggest that, in addition to the availability of the ligand, PDGF-mediated cell growth in vivo is dependent on factors regulating expression of the receptor.",
author = "Louis Terracio and L. Ronnstrand and A. Tingstrom and K. Rubin and L. Claesson-Welsh and K. Funa and Heldin, {C. H.}",
year = "1988",
language = "English (US)",
volume = "107",
pages = "1947--1957",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing

AU - Terracio, Louis

AU - Ronnstrand, L.

AU - Tingstrom, A.

AU - Rubin, K.

AU - Claesson-Welsh, L.

AU - Funa, K.

AU - Heldin, C. H.

PY - 1988

Y1 - 1988

N2 - The expression of platelet-derived growth factor (PDGF) receptors in porcine uterus and human skin in situ, was compared with that of cultured primary cells isolated from the same tissues, PDGF receptor expression was examined by monoclonal antibodies specific for the B type PDGF receptor and by RNA/RNA in situ hybridization with a probe constructed from a cDNA clone encoding the B type PDGF receptor. In porcine uterus tissue both mRNA and the protein product for the PDGF receptor were detected in the endometrium; the myometrium, in contrast, contained much lower amounts. Moreover, freshly isolated myometrial cells were devoid of PDGF receptors. However, after 1 d in culture receptors appeared, and after 2 wk of culturing essentially all of the myometrial cells stained positively with the anti-PDGF receptor antibodies and contained PDGF receptor mRNA. Similarly, B type PDGF receptors were not detected in normal human skin, but fibroblast-like cells from explant cultures of human skin possessed PDGF receptors. When determined by immunoblotting, porcine uterus myometrial membranes contained ~20% of the PDGF receptor antigen compared with the amount found in endometrial membranes. In addition, PDGF stimulated the phosphorylation of a 175-kD component, most likely representing autophosphorylation of the B type of PDGF receptor in endometrial membranes, whereas only a marginal phosphorylation was seen in myometrial membranes. Taken together, these results demonstrate that PDGF receptor expression varies in normal tissues and that fibroblasts and smooth muscle cells do not uniformly express the receptor in situ. Furthermore, fibroblasts and smooth muscle cells that are released from tissues are induced to express PDGF receptors in response to cell culturing. The data suggest that, in addition to the availability of the ligand, PDGF-mediated cell growth in vivo is dependent on factors regulating expression of the receptor.

AB - The expression of platelet-derived growth factor (PDGF) receptors in porcine uterus and human skin in situ, was compared with that of cultured primary cells isolated from the same tissues, PDGF receptor expression was examined by monoclonal antibodies specific for the B type PDGF receptor and by RNA/RNA in situ hybridization with a probe constructed from a cDNA clone encoding the B type PDGF receptor. In porcine uterus tissue both mRNA and the protein product for the PDGF receptor were detected in the endometrium; the myometrium, in contrast, contained much lower amounts. Moreover, freshly isolated myometrial cells were devoid of PDGF receptors. However, after 1 d in culture receptors appeared, and after 2 wk of culturing essentially all of the myometrial cells stained positively with the anti-PDGF receptor antibodies and contained PDGF receptor mRNA. Similarly, B type PDGF receptors were not detected in normal human skin, but fibroblast-like cells from explant cultures of human skin possessed PDGF receptors. When determined by immunoblotting, porcine uterus myometrial membranes contained ~20% of the PDGF receptor antigen compared with the amount found in endometrial membranes. In addition, PDGF stimulated the phosphorylation of a 175-kD component, most likely representing autophosphorylation of the B type of PDGF receptor in endometrial membranes, whereas only a marginal phosphorylation was seen in myometrial membranes. Taken together, these results demonstrate that PDGF receptor expression varies in normal tissues and that fibroblasts and smooth muscle cells do not uniformly express the receptor in situ. Furthermore, fibroblasts and smooth muscle cells that are released from tissues are induced to express PDGF receptors in response to cell culturing. The data suggest that, in addition to the availability of the ligand, PDGF-mediated cell growth in vivo is dependent on factors regulating expression of the receptor.

UR - http://www.scopus.com/inward/record.url?scp=0023737477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023737477&partnerID=8YFLogxK

M3 - Article

VL - 107

SP - 1947

EP - 1957

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -