Induction of neural crest in Xenopus by transcription factor AP2α

Ting Luo, Young Hoon Lee, Jean-Pierre Saint-Jeannet, Thomas D. Sargent

Research output: Contribution to journalArticle

Abstract

We report experiments with Xenopus laevis, using both intact embryos and ectodermal explants, showing that the transcription factor AP2α is positively regulated by bone morphogenetic protein (BMP) and Wnt signaling, and that this activation is an essential step in the induction of neural crest (NC). Ectopic expression of AP2α is sufficient to activate high-level expression of NC-specific genes such as Slug and Sox9, which can occur as isolated domains within the neural plate as well as by expansion of endogenous NC territories. AP2α also has the property of inducing NC in isolated ectoderm in which Wnt signaling is provided but BMP signaling is minimized by overexpression of chordin. Like other NC regulatory factors, activation of AP2α requires some attenuation of endogenous BMP signaling; however, this process occurs at a lower threshold for AP2α. Furthermore, AP2α expression domains are larger than for other NC factors. Loss-of-function experiments with antisense AP2α morpholino oligonucleotides result in severe reduction in the NC territory. These results support a central role for AP2α in NC induction. We propose a model in which AP2α expression, along with inactivation of NC inhibitory factors such as Dlx3, establish a feedback loop comprising AP2α, Sox9, and Slug, leading to and maintaining NC specification.

Original languageEnglish (US)
Pages (from-to)532-537
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number2
DOIs
StatePublished - Jan 21 2003

Fingerprint

Transcription Factor AP-2
Neural Crest
Xenopus
Bone Morphogenetic Proteins
Gastropoda
Neural Plate
Morpholinos
Ectoderm
Xenopus laevis
Embryonic Structures

Keywords

  • Bone morphogenetic protein
  • Morpholino antisense oligonucleotides
  • Slug
  • Sox9
  • Wnt

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Induction of neural crest in Xenopus by transcription factor AP2α. / Luo, Ting; Lee, Young Hoon; Saint-Jeannet, Jean-Pierre; Sargent, Thomas D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 2, 21.01.2003, p. 532-537.

Research output: Contribution to journalArticle

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