Incorporation of Macromolecules into α-Lactose Monohydrate Crystals

Alexander G. Shtukenberg, Kelly Tripathi, Remi Ketchum, Jenny Jaehee Jeon, Ahmed Sanda, Bart Kahr

Research output: Contribution to journalArticle

Abstract

It had been discovered that crystals of α-lactose monohydrate can occlude and give kinetic stability to various proteins, an unexpected observation suggesting a process for storing protein vaccines and biopharmaceuticals at room temperature. The growth of α-lactose monohydrate with proteins is analyzed herein, emphasizing segregation coefficients, the relationships between protein incorporation and buffer composition, temperature, growth rate, and mass transport. Detailed examination of bovine serum albumin is supported by data for eight other proteins. A search for other crystals with lactose-like inclusivity of macromolecular guests is also reported.

Original languageEnglish (US)
Pages (from-to)4589-4598
Number of pages10
JournalCrystal Growth and Design
Volume16
Issue number8
DOIs
StatePublished - Aug 3 2016

Fingerprint

lactose
Lactose
Macromolecules
macromolecules
proteins
Proteins
Crystals
crystals
vaccines
Vaccines
Bovine Serum Albumin
albumins
serums
Buffers
Mass transfer
buffers
examination
Temperature
Kinetics
kinetics

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Shtukenberg, A. G., Tripathi, K., Ketchum, R., Jeon, J. J., Sanda, A., & Kahr, B. (2016). Incorporation of Macromolecules into α-Lactose Monohydrate Crystals. Crystal Growth and Design, 16(8), 4589-4598. https://doi.org/10.1021/acs.cgd.6b00686

Incorporation of Macromolecules into α-Lactose Monohydrate Crystals. / Shtukenberg, Alexander G.; Tripathi, Kelly; Ketchum, Remi; Jeon, Jenny Jaehee; Sanda, Ahmed; Kahr, Bart.

In: Crystal Growth and Design, Vol. 16, No. 8, 03.08.2016, p. 4589-4598.

Research output: Contribution to journalArticle

Shtukenberg, AG, Tripathi, K, Ketchum, R, Jeon, JJ, Sanda, A & Kahr, B 2016, 'Incorporation of Macromolecules into α-Lactose Monohydrate Crystals', Crystal Growth and Design, vol. 16, no. 8, pp. 4589-4598. https://doi.org/10.1021/acs.cgd.6b00686
Shtukenberg, Alexander G. ; Tripathi, Kelly ; Ketchum, Remi ; Jeon, Jenny Jaehee ; Sanda, Ahmed ; Kahr, Bart. / Incorporation of Macromolecules into α-Lactose Monohydrate Crystals. In: Crystal Growth and Design. 2016 ; Vol. 16, No. 8. pp. 4589-4598.
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