Inactivation of viruses in blood with aluminum phthalocyanine derivatives

B. Horowitz, B. Williams, S. Rywkin, A. M. Prince, Nicholas Geacintov, N. Geacintov, J. Valinsky

Research output: Contribution to journalArticle

Abstract

The inactivation of viruses added to whole blood and a red cell concentrate with aluminum phthalocyanine and its sulfonated derivatives was studied. A cell-free form of vesicular stomatitis virus (VSV), used as a model, was completely inactivated (>104 infectious units; TCID50) on treatment of whole blood with 10 μM (10 μmol/L) aluminum phthalocyanine chloride (AIPc) and a visible light dosage of 88 to 176 J per cm2. At 44 J per cm2, complete VSV inactivation was achieved on raising the concentration of AIPc to 25 μM (25 μmol/L). Results at least as good were achieved on similar treatment of a red cell concentrate. Also inactivated were a cell-associated form of VSV and both cell-free and cell-associated forms of human immunodeficiency virus; encephalomyocarditis virus, used as a model for non-lipid-enveloped viruses, was not inactivated by this procedure. This inactivation of cell-free VSV suggests that a similar degree of inactivation could be achieved with a lower concentration of the sulfonated forms of aluminum phthalocyanine. Throughout the above studies, red cell integrity was well maintained, as judged by the absence of hemoglobin release (≤2%) during the course of treatment or on subsequent storage. Red cell osmotic fragility was decreased on treatment of whole blood with AIPc. This study indicates that AIPc may be a promising method for the inactivation of viruses in cellular blood products.

Original languageEnglish (US)
Pages (from-to)102-108
Number of pages7
JournalTransfusion
Volume31
Issue number2
StatePublished - 1991

Fingerprint

Virus Inactivation
Vesicular Stomatitis
Viruses
Osmotic Fragility
Encephalomyocarditis virus
aluminum phthalocyanine
Hemoglobins
Erythrocytes
HIV
Light
chloroaluminum phthalocyanine

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Horowitz, B., Williams, B., Rywkin, S., Prince, A. M., Geacintov, N., Geacintov, N., & Valinsky, J. (1991). Inactivation of viruses in blood with aluminum phthalocyanine derivatives. Transfusion, 31(2), 102-108.

Inactivation of viruses in blood with aluminum phthalocyanine derivatives. / Horowitz, B.; Williams, B.; Rywkin, S.; Prince, A. M.; Geacintov, Nicholas; Geacintov, N.; Valinsky, J.

In: Transfusion, Vol. 31, No. 2, 1991, p. 102-108.

Research output: Contribution to journalArticle

Horowitz, B, Williams, B, Rywkin, S, Prince, AM, Geacintov, N, Geacintov, N & Valinsky, J 1991, 'Inactivation of viruses in blood with aluminum phthalocyanine derivatives', Transfusion, vol. 31, no. 2, pp. 102-108.
Horowitz B, Williams B, Rywkin S, Prince AM, Geacintov N, Geacintov N et al. Inactivation of viruses in blood with aluminum phthalocyanine derivatives. Transfusion. 1991;31(2):102-108.
Horowitz, B. ; Williams, B. ; Rywkin, S. ; Prince, A. M. ; Geacintov, Nicholas ; Geacintov, N. ; Valinsky, J. / Inactivation of viruses in blood with aluminum phthalocyanine derivatives. In: Transfusion. 1991 ; Vol. 31, No. 2. pp. 102-108.
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