In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics

Jia Xuan Chen, Patricia G. Cipriani, Desirea Mecenas, Jolanta Polanowska, Fabio Piano, Kristin C. Gunsalus, Matthias Selbach

Research output: Contribution to journalArticle

Abstract

Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. Elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.

Original languageEnglish (US)
Pages (from-to)1642-1657
Number of pages16
JournalMolecular and Cellular Proteomics
Volume15
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

Caenorhabditis elegans
Proteomics
Embryonic Structures
Proteins
Protein Interaction Maps
Phosphorylation
Embryonic Development
Phosphotransferases
Purification
Maintenance
Animals
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics. / Chen, Jia Xuan; Cipriani, Patricia G.; Mecenas, Desirea; Polanowska, Jolanta; Piano, Fabio; Gunsalus, Kristin C.; Selbach, Matthias.

In: Molecular and Cellular Proteomics, Vol. 15, No. 5, 01.05.2016, p. 1642-1657.

Research output: Contribution to journalArticle

Chen, Jia Xuan ; Cipriani, Patricia G. ; Mecenas, Desirea ; Polanowska, Jolanta ; Piano, Fabio ; Gunsalus, Kristin C. ; Selbach, Matthias. / In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics. In: Molecular and Cellular Proteomics. 2016 ; Vol. 15, No. 5. pp. 1642-1657.
@article{12a38ca7f7da407bb9d17b22a38e10ff,
title = "In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics",
abstract = "Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. Elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.",
author = "Chen, {Jia Xuan} and Cipriani, {Patricia G.} and Desirea Mecenas and Jolanta Polanowska and Fabio Piano and Gunsalus, {Kristin C.} and Matthias Selbach",
year = "2016",
month = "5",
day = "1",
doi = "10.1074/mcp.M115.053975",
language = "English (US)",
volume = "15",
pages = "1642--1657",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

TY - JOUR

T1 - In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics

AU - Chen, Jia Xuan

AU - Cipriani, Patricia G.

AU - Mecenas, Desirea

AU - Polanowska, Jolanta

AU - Piano, Fabio

AU - Gunsalus, Kristin C.

AU - Selbach, Matthias

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. Elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.

AB - Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. Elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.

UR - http://www.scopus.com/inward/record.url?scp=84964720811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964720811&partnerID=8YFLogxK

U2 - 10.1074/mcp.M115.053975

DO - 10.1074/mcp.M115.053975

M3 - Article

C2 - 26912668

AN - SCOPUS:84964720811

VL - 15

SP - 1642

EP - 1657

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 5

ER -