In vitro and in vivo biocompatibility of ReOss® in powder and putty configurations

Andréa Vaz Braga Pintor, Rodrigo Figueiredo de Brito Resende, Adriana Terezinha Novelino Neves, Gutemberg Gomes Alves, Paulo Coelho, José Mauro Granjeiro, Monica Diuana Calasans-Maia

Research output: Contribution to journalArticle

Abstract

This study evaluated comparatively two configurations (powder and putty) of a composite biomaterial based on PLGA (Poly(lactide-co-glycolide)/nanoescale hydroxyapatite (ReOss®, Intra-Lock International) through microscopic morphology, in vitro cytotoxicity, biocompatibility and in vivo response as a bone substitute. SEM and EDS characterized the biomaterials before/after grafting. Cytocompatibility was assessed with murine pre-osteoblasts. Osteoconductivity and biocompatibility were evaluated in White New Zealand rabbits. Both configurations were implanted in the calvaria of eighteen animals in non-critical size defects, with blood clot as the control group. After 30, 60 and 90 days, the animals were euthanized and the fragments containing the biomaterials and controls were harvested. Bone blocks were embedded in paraffin (n=15) aiming at histological and histomorphometric analysis, and in resin (n=3) aiming at SEM and EDS. Before implantation, the putty configuration showed both a porous and a fibrous morphological phase. Powder revealed porous particles with variable granulometry. EDS showed calcium, carbon, and oxygen in putty configuration, while powder also showed phosphorus. After implantation EDS revealed calcium, carbon, and oxygen in both configurations. The materials were considered cytotoxic by the XTT test. Histological analysis showed new bone formation and no inflammatory reaction at implant sites. However, the histomorphometric analysis indicated that the amount of newly formed bone was not statistically different between experimental groups. Although both materials presented in vitro cytotoxicity, they were biocompatible and osteoconductive. The configuration of ReOss® affected morphological characteristics and the in vitro cytocompatibility but did not impact on the in vivo biological response, as measured by the present model.

Original languageEnglish (US)
Pages (from-to)117-127
Number of pages11
JournalBrazilian Dental Journal
Volume29
Issue number2
DOIs
StatePublished - Mar 1 2018

Fingerprint

Biocompatible Materials
Powders
Carbon
Oxygen
Calcium
Bone Substitutes
Polyglactin 910
Bone and Bones
Durapatite
Osteoblasts
Osteogenesis
Skull
Paraffin
Phosphorus
Thrombosis
Rabbits
Control Groups
In Vitro Techniques

Keywords

  • Biocompatibility testing
  • Hydroxyapatite
  • Materials testing
  • Nanotechnology
  • PLGA

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Pintor, A. V. B., Resende, R. F. D. B., Neves, A. T. N., Alves, G. G., Coelho, P., Granjeiro, J. M., & Calasans-Maia, M. D. (2018). In vitro and in vivo biocompatibility of ReOss® in powder and putty configurations. Brazilian Dental Journal, 29(2), 117-127. https://doi.org/10.1590/0103-6440201802017

In vitro and in vivo biocompatibility of ReOss® in powder and putty configurations. / Pintor, Andréa Vaz Braga; Resende, Rodrigo Figueiredo de Brito; Neves, Adriana Terezinha Novelino; Alves, Gutemberg Gomes; Coelho, Paulo; Granjeiro, José Mauro; Calasans-Maia, Monica Diuana.

In: Brazilian Dental Journal, Vol. 29, No. 2, 01.03.2018, p. 117-127.

Research output: Contribution to journalArticle

Pintor, AVB, Resende, RFDB, Neves, ATN, Alves, GG, Coelho, P, Granjeiro, JM & Calasans-Maia, MD 2018, 'In vitro and in vivo biocompatibility of ReOss® in powder and putty configurations', Brazilian Dental Journal, vol. 29, no. 2, pp. 117-127. https://doi.org/10.1590/0103-6440201802017
Pintor, Andréa Vaz Braga ; Resende, Rodrigo Figueiredo de Brito ; Neves, Adriana Terezinha Novelino ; Alves, Gutemberg Gomes ; Coelho, Paulo ; Granjeiro, José Mauro ; Calasans-Maia, Monica Diuana. / In vitro and in vivo biocompatibility of ReOss® in powder and putty configurations. In: Brazilian Dental Journal. 2018 ; Vol. 29, No. 2. pp. 117-127.
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