In vitro analysis of position- and lineage-dependent selectivity in the formation of neuromuscular synapses

Dan Sanes, Mu ming Poot

Research output: Contribution to journalArticle

Abstract

The hypotheses that selective formation of nerve-muscle connections depends upon intrinsic cellular properties, endowed either by the cell's rostral-caudal position in the embryo or its lineage, were tested directly in Xenopus embryonic cell cultures. The position or the lineage of embryonic cells was traced in vitro by previous injection of fluorophore-conjugated dextran molecules into individual blastomeres. Synaptic efficacy was assayed by recording synaptic currents from neurite-contacted muscle cells in the culture, and the physical affinity of neurites for muscle cells of different positional or clonal origins was assayed by counting the frequency of association between the neurites' growth cones and the muscle cells. Both assays showed no apparent preference between nerve and muscle cells of similar rostral-caudal positions or clonal origins, suggesting that there is little position- or lineage-dependent selectivity in the initial nerve-muscle interactions.

Original languageEnglish (US)
Pages (from-to)1237-1244
Number of pages8
JournalNeuron
Volume2
Issue number3
DOIs
StatePublished - 1989

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Synapses
Muscle Cells
Neurites
Cell Culture Techniques
Blastomeres
Muscles
Growth Cones
Xenopus
Dextrans
Embryonic Structures
Neurons
Injections
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In vitro analysis of position- and lineage-dependent selectivity in the formation of neuromuscular synapses. / Sanes, Dan; Poot, Mu ming.

In: Neuron, Vol. 2, No. 3, 1989, p. 1237-1244.

Research output: Contribution to journalArticle

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