In situ chromatin-binding assay using epifluorescent microscopy in S. pombe

Jinpu Yang, Fei Li

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chromatin-associated proteins play critical roles in many cellular processes, including gene expression, epigenetic regulation, DNA repair, recombination, and replication. Especially, epigenetic landscape, shaped by a variety of chromatin-binding proteins, is dynamic and regulated in a context-dependent manner. In situ chromatin-binding assay is a powerful but simple tool to investigate how proteins, such as epigenetic components, associate with chromatin. This approach relies on the fact that chromatin bound proteins are more resistant to detergent extraction. Here, we describe a protocol for the in situ chromatin-binding assay used in Schizosaccaromyces pombe.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-165
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1721
ISSN (Print)1064-3745

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Keywords

  • CENP-A
  • Epigenetics
  • Fission yeast
  • GFP
  • H3
  • In situ chromatin binding
  • Schizosaccaromyces pombe

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yang, J., & Li, F. (2018). In situ chromatin-binding assay using epifluorescent microscopy in S. pombe. In Methods in Molecular Biology (pp. 155-165). (Methods in Molecular Biology; Vol. 1721). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7546-4_14