In silico reconstitution of actin-based symmetry breaking and motility

Mark J. Dayel, Orkun Akin, Mark Landeryou, Viviana Risca, Alex Mogilner, R. Dyche Mullins

Research output: Contribution to journalArticle

Abstract

Eukaryotic cells assemble viscoelastic networks of crosslinked actin filaments to control their shape, mechanical properties, and motility. One important class of actin network is nucleated by the Arp2/3 complex and drives both membrane protrusion at the leading edge of motile cells and intracellular motility of pathogens such as Listeria monocytogenes. These networks can be reconstituted in vitro from purified components to drive the motility of spherical micron-sized beads. An Elastic Gel model has been successful in explaining how these networks break symmetry, but how they produce directed motile force has been less clear. We have combined numerical simulations with in vitro experiments to reconstitute the behavior of these motile actin networks in silico using an Accumulative Particle-Spring (APS) model that builds on the Elastic Gel model, and demonstrates simple intuitive mechanisms for both symmetry breaking and sustained motility. The APS model explains observed transitions between smooth and pulsatile motion as well as subtle variations in network architecture caused by differences in geometry and conditions. Our findings also explain sideways symmetry breaking and motility of elongated beads, and show that elastic recoil, though important for symmetry breaking and pulsatile motion, is not necessary for smooth directional motility. The APS model demonstrates how a small number of viscoelastic network parameters and construction rules suffice to recapture the complex behavior of motile actin networks. The fact that the model not only mirrors our in vitro observations, but also makes novel predictions that we confirm by experiment, suggests that the model captures much of the essence of actin-based motility in this system.

Original languageEnglish (US)
Article numbere1000201
JournalPLoS Biology
Volume7
Issue number9
DOIs
StatePublished - 2009

Fingerprint

Computer Simulation
actin
Actins
Gels
Actin-Related Protein 2-3 Complex
Listeria monocytogenes
Eukaryotic Cells
Actin Cytoskeleton
Cell Movement
gels
Listeria
Pathogens
Membranes
microfilaments
Network architecture
mechanical properties
eukaryotic cells
In Vitro Techniques
Experiments
Mechanical properties

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Dayel, M. J., Akin, O., Landeryou, M., Risca, V., Mogilner, A., & Mullins, R. D. (2009). In silico reconstitution of actin-based symmetry breaking and motility. PLoS Biology, 7(9), [e1000201]. https://doi.org/10.1371/journal.pbio.1000201

In silico reconstitution of actin-based symmetry breaking and motility. / Dayel, Mark J.; Akin, Orkun; Landeryou, Mark; Risca, Viviana; Mogilner, Alex; Mullins, R. Dyche.

In: PLoS Biology, Vol. 7, No. 9, e1000201, 2009.

Research output: Contribution to journalArticle

Dayel, MJ, Akin, O, Landeryou, M, Risca, V, Mogilner, A & Mullins, RD 2009, 'In silico reconstitution of actin-based symmetry breaking and motility', PLoS Biology, vol. 7, no. 9, e1000201. https://doi.org/10.1371/journal.pbio.1000201
Dayel, Mark J. ; Akin, Orkun ; Landeryou, Mark ; Risca, Viviana ; Mogilner, Alex ; Mullins, R. Dyche. / In silico reconstitution of actin-based symmetry breaking and motility. In: PLoS Biology. 2009 ; Vol. 7, No. 9.
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