In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control

Xin Xie, Tomas Venit, Nizar Drou, Piergiorgio Percipalle

Research output: Contribution to journalArticle

Abstract

In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO)β-actin −/− mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP)in β-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA)transcription and nuclear oxidative phosphorylation (OXPHOS)gene expression. Using super-resolution microscopy we provided direct evidence on the presence of β-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without β-actin. The observation that mitochondria-targeted β-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial β-actin pool necessary for mitochondrial quality control.

Original languageEnglish (US)
Pages (from-to)226-237
Number of pages12
JournalFood Science and Human Wellness
Volume3
DOIs
StatePublished - May 25 2018

Fingerprint

Mitochondrial DNA
Quality Control
actin
quality control
Actins
Mitochondria
mitochondria
mitochondrial DNA
transcription (genetics)
Mitochondrial Membrane Potential
membrane potential
Null Lymphocytes
oxidative phosphorylation
Oxidative Phosphorylation
Eukaryotic Cells
cell aggregates
Knockout Mice
bulbs
fibroblasts
eukaryotic cells

Keywords

  • Cell Biology
  • Functional Aspects of Cell Biology
  • Molecular Biology

ASJC Scopus subject areas

  • Food Science
  • General

Cite this

In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control. / Xie, Xin; Venit, Tomas; Drou, Nizar; Percipalle, Piergiorgio.

In: Food Science and Human Wellness, Vol. 3, 25.05.2018, p. 226-237.

Research output: Contribution to journalArticle

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AB - In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO)β-actin −/− mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP)in β-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA)transcription and nuclear oxidative phosphorylation (OXPHOS)gene expression. Using super-resolution microscopy we provided direct evidence on the presence of β-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without β-actin. The observation that mitochondria-targeted β-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial β-actin pool necessary for mitochondrial quality control.

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