In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control

Xin Xie, Tomas Venit, Nizar Drou, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO)β-actin −/− mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP)in β-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA)transcription and nuclear oxidative phosphorylation (OXPHOS)gene expression. Using super-resolution microscopy we provided direct evidence on the presence of β-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without β-actin. The observation that mitochondria-targeted β-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial β-actin pool necessary for mitochondrial quality control.

    Original languageEnglish (US)
    Pages (from-to)226-237
    Number of pages12
    JournalFood Science and Human Wellness
    Volume3
    DOIs
    StatePublished - May 25 2018

    Fingerprint

    Mitochondrial DNA
    Quality Control
    actin
    quality control
    Actins
    Mitochondria
    mitochondria
    mitochondrial DNA
    transcription (genetics)
    Mitochondrial Membrane Potential
    membrane potential
    Null Lymphocytes
    oxidative phosphorylation
    Oxidative Phosphorylation
    Eukaryotic Cells
    cell aggregates
    Knockout Mice
    bulbs
    fibroblasts
    eukaryotic cells

    Keywords

    • Cell Biology
    • Functional Aspects of Cell Biology
    • Molecular Biology

    ASJC Scopus subject areas

    • Food Science
    • General

    Cite this

    In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control. / Xie, Xin; Venit, Tomas; Drou, Nizar; Percipalle, Piergiorgio.

    In: Food Science and Human Wellness, Vol. 3, 25.05.2018, p. 226-237.

    Research output: Contribution to journalArticle

    Xie, Xin ; Venit, Tomas ; Drou, Nizar ; Percipalle, Piergiorgio. / In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control. In: Food Science and Human Wellness. 2018 ; Vol. 3. pp. 226-237.
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    abstract = "In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO)β-actin −/− mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP)in β-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA)transcription and nuclear oxidative phosphorylation (OXPHOS)gene expression. Using super-resolution microscopy we provided direct evidence on the presence of β-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without β-actin. The observation that mitochondria-targeted β-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial β-actin pool necessary for mitochondrial quality control.",
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