In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences

Chandrasekhar S. Raju, Christian Göritz, Ylva Nord, Ola Hermanson, Carmen López-Iglesias, Neus Visa, Goncalo Castelo-Branco, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    Heterogeneous ribonucleoproteins (hnRNPs) have key roles in RNA biogenesis, including pre-mRNP assembly, transport and cytoplasmic localization. Here we show by biochemical fractionation of nuclear extracts and protein-protein interaction assays that the A/B-type hnRNP CBF-A is in a multiprotein complex with hnRNP A2 and A3 and hnRNP U. Using RNA affinity chromatography and gel retardation assays, CBF-A was found to bind directly to RNA trafficking sequences in the 3′-UTR of the myelin basic protein (MBP) mRNA. In primary oligodendrocytes, astrocytes, neurons, and mouse forebrain sections, CBF-A revealed a characteristic granular cytoplasmic distribution. In mouse forebrain CBF-A-positive granules were preferentially found in regions with loosely bundled myelin fibers. In cultured oligodendrocytes, CBF-A was found to be specifically associated with endogenous MBP mRNA and CBF-A gene silencing resulted in the retention of MBP granules in the cell body. Finally, immunoelectron microscopy in differentiating oligodendrocytes showed that CBF-A is located in cytoplasmic granules that are often associated with the cytoskeleton. The results suggest that CBF-A is a novel transacting factor required for cytoplasmic mRNA transport and localization.

    Original languageEnglish (US)
    Pages (from-to)3008-3019
    Number of pages12
    JournalMolecular Biology of the Cell
    Volume19
    Issue number7
    DOIs
    StatePublished - Jul 1 2008

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    Heterogeneous-Nuclear Ribonucleoproteins
    Myelin Basic Protein
    Oligodendroglia
    Prosencephalon
    Messenger RNA
    Heterogeneous-Nuclear Ribonucleoprotein U
    RNA
    Cytoplasmic Granules
    Multiprotein Complexes
    Ribonucleoproteins
    Immunoelectron Microscopy
    3' Untranslated Regions
    Gene Silencing
    Electrophoretic Mobility Shift Assay
    Myelin Sheath
    Nuclear Proteins
    Cytoskeleton
    Affinity Chromatography
    Astrocytes
    Neurons

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

    Cite this

    Raju, C. S., Göritz, C., Nord, Y., Hermanson, O., López-Iglesias, C., Visa, N., ... Percipalle, P. (2008). In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences. Molecular Biology of the Cell, 19(7), 3008-3019. https://doi.org/10.1091/mbc.E07-10-1083

    In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences. / Raju, Chandrasekhar S.; Göritz, Christian; Nord, Ylva; Hermanson, Ola; López-Iglesias, Carmen; Visa, Neus; Castelo-Branco, Goncalo; Percipalle, Piergiorgio.

    In: Molecular Biology of the Cell, Vol. 19, No. 7, 01.07.2008, p. 3008-3019.

    Research output: Contribution to journalArticle

    Raju, CS, Göritz, C, Nord, Y, Hermanson, O, López-Iglesias, C, Visa, N, Castelo-Branco, G & Percipalle, P 2008, 'In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences', Molecular Biology of the Cell, vol. 19, no. 7, pp. 3008-3019. https://doi.org/10.1091/mbc.E07-10-1083
    Raju, Chandrasekhar S. ; Göritz, Christian ; Nord, Ylva ; Hermanson, Ola ; López-Iglesias, Carmen ; Visa, Neus ; Castelo-Branco, Goncalo ; Percipalle, Piergiorgio. / In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 7. pp. 3008-3019.
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