Impact of bacterial vaginosis, as assessed by nugent criteria and hormonal status on glycosidases and lectin binding in cervicovaginal lavage samples

Bernard J. Moncla, Catherine A. Chappell, Lara Mahal, Brian M. Debo, Leslie A. Meyn, Sharon L. Hillier

Research output: Contribution to journalArticle

Abstract

The objective of this study was to evaluate the impact of hormonal status and bacterial vaginosis (BV) on the glycosidases present and glycosylation changes as assessed by lectin binding to cervicovaginal lavage constituents. Frozen cervicovaginal lavage samples from a completed study examining the impact of reproductive hormones on the physicochemical properties of vaginal fluid were utilized for the present study. In the parent study, 165 women were characterized as having BV, intermediate or normal microflora using the Nugent criteria. The presence of glycosidases in the samples was determined using quantitative 4-methyl-umbelliferone based assays, and glycosylation was assessed using enzyme linked lectin assays (ELLA). Women with BV had elevated sialidase, α-galactosidase, β-galactosidase and α-glucosidase activities compared to intermediate or normal women (P

Original languageEnglish (US)
Article numbere0127091
JournalPLoS One
Volume10
Issue number5
DOIs
StatePublished - May 1 2015

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Galactosidases
Glycosylation
Bacterial Vaginosis
7-hydroxycoumarin
glycosidases
Glycoside Hydrolases
Therapeutic Irrigation
Lectins
lectins
galactosidases
Assays
glycosylation
Glucosidases
Neuraminidase
glucosidases
sialidase
assays
Hormones
sampling
Fluids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Impact of bacterial vaginosis, as assessed by nugent criteria and hormonal status on glycosidases and lectin binding in cervicovaginal lavage samples. / Moncla, Bernard J.; Chappell, Catherine A.; Mahal, Lara; Debo, Brian M.; Meyn, Leslie A.; Hillier, Sharon L.

In: PLoS One, Vol. 10, No. 5, e0127091, 01.05.2015.

Research output: Contribution to journalArticle

Moncla, Bernard J. ; Chappell, Catherine A. ; Mahal, Lara ; Debo, Brian M. ; Meyn, Leslie A. ; Hillier, Sharon L. / Impact of bacterial vaginosis, as assessed by nugent criteria and hormonal status on glycosidases and lectin binding in cervicovaginal lavage samples. In: PLoS One. 2015 ; Vol. 10, No. 5.
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