Immunofluorescent localization of an androgen-dependent isoenzyme of prostatic acid phosphatase and rat ventral prostate

Louis Terracio, A. Rule, J. Salvato, W. H J Douglas

Research output: Contribution to journalArticle

Abstract

Isoenzymes of rat ventral prostate (RVP) acid phosphatase were isolated and partially purified by ultracentrifugation, Sephadex G-100 column chromatography, and isoelectric focusing. Antisera were raised to the isoenzymes of prostatic acid phosphatase by immunization of New Zealand white rabbits. Rabbit antisera reacting specifically to homologous but not heterologous isoenzymes of acid phosphatase were then reacted with a variety of tissues using indirect immunofluorescence. The tissues included prostate, spleen, bone marrow, liver, kidney, salivary gland complex, small intestine, and adrenal glands. An antiserum against a RVP acid phosphatase isoenzyme with a pI of 4.5 (A-PAP) localized acid phosphatase only in the supranuclear region of rat ventral prostate epithelial cells, and did not react with acid phosphatase in any of the other organs tested. A-PAP did not localize acid phosphatase in the ventral prostate from rats 14 days after castration. A-PAP did localize acid phosphatase in the ventral prostate from castrated animals that were treated with testosterone. These results indicate that A-PAP localized an androgen-dependent isoenzyme of acid phosphatase in RVP epithelial cells that may be secretory in nature. This antiserum should prove to be an ideal marker for studies involving hormonal regulation of prostatic epithelial function in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)131-139
Number of pages9
JournalAnatomical Record
Volume213
Issue number2
StatePublished - 1985

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androgen
Acid Phosphatase
androgens
acid phosphatase
phosphatase
Androgens
Isoenzymes
isozymes
Prostate
Immune Sera
acid
rats
antiserum
Epithelial Cells
Rabbits
Ultracentrifugation
Castration
Isoelectric Focusing
epithelial cells
Indirect Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Immunofluorescent localization of an androgen-dependent isoenzyme of prostatic acid phosphatase and rat ventral prostate. / Terracio, Louis; Rule, A.; Salvato, J.; Douglas, W. H J.

In: Anatomical Record, Vol. 213, No. 2, 1985, p. 131-139.

Research output: Contribution to journalArticle

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