Immunocytochemical study of GnRH and GnRH-associated peptide in male Syrian hamsters as a function of photoperiod and gonadal alterations

E. Ronchi, Chiye Aoki, L. C. Krey, D. W. Pfaff

Research output: Contribution to journalArticle

Abstract

Hypothalamic luteinizing-hormone-releasing hormone (GnRH) and gonado-tropin-releasing-hormone-associated peptide (GAP) biosynthesis and storage were estimated by immunocytochemistry in male golden hamsters maintained in different photoperiods. Intact or castrated male hamsters with subcutaneously inserted testosterone implants were exposed to long-day (14:10) or short-day photoperiods (10:14) for 4-8 weeks. Exposure to short photoperiod for 4 weeks, an interval characterized by a suppression of gonadotropin secretion but not gonadal regression, was associated with an increase in the number of GnRH- and GAP-immunoreactive cells in the diagonal band of Broca/medial septum. Furthermore, morphometric analysis revealed that these animals displayed significantly more GnRH but not GAP immunoreactivity in the median eminence as opposed to hamsters exposed to long-day photoperiods. In additional studies, gonadally regressed hamsters exposed to short day lengths for 8 weeks had equal numbers of GnRH cells as did the long-day controls. These patterns suggest that reproductive quiescence in golden hamsters is not the result of depletions of neuronal GnRH stores available for secretion.

Original languageEnglish (US)
Pages (from-to)134-145
Number of pages12
JournalNeuroendocrinology
Volume55
Issue number2
StatePublished - 1992

Fingerprint

Mesocricetus
Photoperiod
Gonadotropin-Releasing Hormone
Cricetinae
Peptide Biosynthesis
Diagonal Band of Broca
Hypothalamic Hormones
Median Eminence
Gonadotropins
Testosterone
gonadotropin releasing hormone associated peptide
Cell Count
Immunohistochemistry
Hormones

Keywords

  • Gonadal regression
  • Immunocytochemistry
  • Luteinizing-hormone-releasing hormone
  • Luteinizing-hormone-releasing-hormone-associated peptide
  • Photoperiod

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Immunocytochemical study of GnRH and GnRH-associated peptide in male Syrian hamsters as a function of photoperiod and gonadal alterations. / Ronchi, E.; Aoki, Chiye; Krey, L. C.; Pfaff, D. W.

In: Neuroendocrinology, Vol. 55, No. 2, 1992, p. 134-145.

Research output: Contribution to journalArticle

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abstract = "Hypothalamic luteinizing-hormone-releasing hormone (GnRH) and gonado-tropin-releasing-hormone-associated peptide (GAP) biosynthesis and storage were estimated by immunocytochemistry in male golden hamsters maintained in different photoperiods. Intact or castrated male hamsters with subcutaneously inserted testosterone implants were exposed to long-day (14:10) or short-day photoperiods (10:14) for 4-8 weeks. Exposure to short photoperiod for 4 weeks, an interval characterized by a suppression of gonadotropin secretion but not gonadal regression, was associated with an increase in the number of GnRH- and GAP-immunoreactive cells in the diagonal band of Broca/medial septum. Furthermore, morphometric analysis revealed that these animals displayed significantly more GnRH but not GAP immunoreactivity in the median eminence as opposed to hamsters exposed to long-day photoperiods. In additional studies, gonadally regressed hamsters exposed to short day lengths for 8 weeks had equal numbers of GnRH cells as did the long-day controls. These patterns suggest that reproductive quiescence in golden hamsters is not the result of depletions of neuronal GnRH stores available for secretion.",
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