Imaging linear birefringence and dichroism in cerebral amyloid pathologies

Lee Way Jin, Kacey A. Claborn, Miki Kurimoto, Morten A. Geday, Izumi Maezawa, Faranak Sohraby, Marcus Estrada, Werner Kaminksy, Bart Kahr

Research output: Contribution to journalArticle

Abstract

New advances in polarized light microscopy were used to image Congo red-stained cerebral amyloidosis in sharp relief. The rotating-polarizer method was used to separate the optical effects of transmission, linear birefringence, extinction, linear dichroism, and orientation of the electric dipole transition moments and to display them as false-color maps. These effects are typically convolved in an ordinary polarized light microscope. In this way, we show that the amyloid deposits in Alzheimer's disease plaques contain structurally disordered centers, providing clues to mechanisms of crystallization of amyloid in vivo. Comparisons are made with plaques from tissues of subjects having Down's syndrome and a prion disease. In plaques characteristic of each disease, the Congo red molecules are oriented radially. The optical orientation in amyloid deposited in blood vessels from subjects having cerebral amyloid angiopathy was 90° out of phase from that in the plaques, suggesting that the fibrils run tangentially with respect to the circumference of the blood vessels. Our result supports an early model in which Congo red molecules are aligned along the long fiber axis and is in contrast to the most recent binding models that are based on computation. This investigation illustrates that the latest methods for the optical analysis of heterogeneous substances are useful for in situ study of amyloid.

Original languageEnglish (US)
Pages (from-to)15294-15298
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number26
DOIs
StatePublished - Dec 23 2003

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Birefringence
Congo Red
Amyloid
Pathology
Blood Vessels
Cerebral Amyloid Angiopathy
Polarization Microscopy
Prion Diseases
Amyloid Plaques
Down Syndrome
Crystallization
Alzheimer Disease
Color
Light

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Imaging linear birefringence and dichroism in cerebral amyloid pathologies. / Jin, Lee Way; Claborn, Kacey A.; Kurimoto, Miki; Geday, Morten A.; Maezawa, Izumi; Sohraby, Faranak; Estrada, Marcus; Kaminksy, Werner; Kahr, Bart.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 26, 23.12.2003, p. 15294-15298.

Research output: Contribution to journalArticle

Jin, LW, Claborn, KA, Kurimoto, M, Geday, MA, Maezawa, I, Sohraby, F, Estrada, M, Kaminksy, W & Kahr, B 2003, 'Imaging linear birefringence and dichroism in cerebral amyloid pathologies', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 26, pp. 15294-15298. https://doi.org/10.1073/pnas.2534647100
Jin, Lee Way ; Claborn, Kacey A. ; Kurimoto, Miki ; Geday, Morten A. ; Maezawa, Izumi ; Sohraby, Faranak ; Estrada, Marcus ; Kaminksy, Werner ; Kahr, Bart. / Imaging linear birefringence and dichroism in cerebral amyloid pathologies. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 26. pp. 15294-15298.
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