IL-12 promotes enhanced recovery from vesicular stomatitis virus infection of the central nervous system

Z. Bi, P. Quandt, T. Komatsu, M. Barna, C. S. Reiss

Research output: Contribution to journalArticle

Abstract

To investigate the role of a cytokine in host defense against the vesicular stomatitis virus (VSV) infection of the central nervous system (CNS), IL-12 was injected i.p. into groups of 10 BALB/c mice on days -1, 0, 1, 2, and 3 postinfection. Four days postinfection, mice were examined. IL- 12 strongly enhanced immunity to VSV infection in the CNS as demonstrated by 1) decreased VSV titers in brain homogenate of IL-12-injected mice compared with those of controls; 2) increased expression of inducible nitric oxide synthase in the CNS; 3) enhanced expression of both MHC class I and class II Ags in the CNS; 4) increased T cell infiltration in the CNS, especially in the olfactory bulb; and 5) diminished VSV-induced apoptosis in olfactory bulb. No detrimental effect was observed even with the 200 ng/mouse dose of IL-12. Protective effects of IL-12 were dose dependent. Collectively, these results demonstrate that exogenously added IL-12, even when injected peripherally, significantly enhances recovery from VSV infection of the CNS.

Original languageEnglish (US)
Pages (from-to)5684-5689
Number of pages6
JournalJournal of Immunology
Volume155
Issue number12
StatePublished - 1995

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Vesicular Stomatitis
Virus Diseases
Interleukin-12
Central Nervous System
Olfactory Bulb
Nitric Oxide Synthase Type II
Viral Load
Immunity
Apoptosis
Cytokines
Viruses
T-Lymphocytes
Brain

ASJC Scopus subject areas

  • Immunology

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IL-12 promotes enhanced recovery from vesicular stomatitis virus infection of the central nervous system. / Bi, Z.; Quandt, P.; Komatsu, T.; Barna, M.; Reiss, C. S.

In: Journal of Immunology, Vol. 155, No. 12, 1995, p. 5684-5689.

Research output: Contribution to journalArticle

Bi, Z. ; Quandt, P. ; Komatsu, T. ; Barna, M. ; Reiss, C. S. / IL-12 promotes enhanced recovery from vesicular stomatitis virus infection of the central nervous system. In: Journal of Immunology. 1995 ; Vol. 155, No. 12. pp. 5684-5689.
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