Identification of Pax3 and Zic1 targets in the developing neural crest

Chang Joon Bae, Byung Yong Park, Young Hoon Lee, John W. Tobias, Chang Soo Hong, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

The neural crest (NC) is a multipotent population of migratory cells unique to the vertebrate embryo, contributing to the development of multiple organ systems. Transcription factors pax3 and zic1 are among the earliest genes activated in NC progenitors, and they are both necessary and sufficient to promote NC fate. In order to further characterize the function of these transcription factors during NC development we have used hormone inducible fusion proteins in a Xenopus animal cap assay, and DNA microarray to identify downstream targets of Pax3 and Zic1. Here we present the results of this screen and the initial validation of these targets using quantitative RT-PCR, in situ hybridization and morpholinos-mediated knockdown. Among the targets identified we found several well-characterized NC-specific genes, including snail2, foxd3, gbx2, twist, sox8 and sox9, which validate our approach. We also obtained several factors with no known function in Xenopus NC, which represent novel regulators of NC fate. The comprehensive characterization of Pax3 and Zic1 targets function in the NC gene regulatory network, are essential to understanding the mechanisms regulating the emergence of this important cell population.

Original languageEnglish (US)
Pages (from-to)473-483
Number of pages11
JournalDevelopmental Biology
Volume386
Issue number2
DOIs
StatePublished - Feb 15 2014

Fingerprint

Neural Crest
Xenopus
Transcription Factors
Morpholinos
Gene Regulatory Networks
Oligonucleotide Array Sequence Analysis
Population
Genes
In Situ Hybridization
Vertebrates
Embryonic Structures
Hormones
Polymerase Chain Reaction

Keywords

  • Gene regulatory network
  • Microarray
  • Neural crest
  • Pax3
  • Xenopus
  • Zic1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Identification of Pax3 and Zic1 targets in the developing neural crest. / Bae, Chang Joon; Park, Byung Yong; Lee, Young Hoon; Tobias, John W.; Hong, Chang Soo; Saint-Jeannet, Jean-Pierre.

In: Developmental Biology, Vol. 386, No. 2, 15.02.2014, p. 473-483.

Research output: Contribution to journalArticle

Bae, Chang Joon ; Park, Byung Yong ; Lee, Young Hoon ; Tobias, John W. ; Hong, Chang Soo ; Saint-Jeannet, Jean-Pierre. / Identification of Pax3 and Zic1 targets in the developing neural crest. In: Developmental Biology. 2014 ; Vol. 386, No. 2. pp. 473-483.
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