Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions

Tingting Jiang, Ramya Raviram, Valentina Snetkova, Pedro P. Rocha, Charlotte Proudhon, Sana Badri, Richard Bonneau, Jane A. Skok, Yuval Kluger

Research output: Contribution to journalArticle

Abstract

Use of low resolution single cell DNA FISH and population based high resolution chromosome conformation capture techniques have highlighted the importance of pairwise chromatin interactions in gene regulation. However, it is unlikely that associations involving regulatory elements act in isolation of other interacting partners that also influence their impact. Indeed, the influence of multi-loci interactions remains something of an enigma as beyond low-resolution DNA FISH we do not have the appropriate tools to analyze these. Here we present a method that uses standard 4C-seq data to identify multi-loci interactions from the same cell. We demonstrate the feasibility of our method using 4C-seq data sets that identify known pairwise and novel tri-loci interactions involving the Tcrb and Igk antigen receptor enhancers. We further show that the three Igk enhancers, MiEκ, 3'Eκ and Edκ, interact simultaneously in this super-enhancer cluster, which add to our previous findings showing that loss of one element decreases interactions between all three elements as well as reducing their transcriptional output. These findings underscore the functional importance of simultaneous interactions and provide new insight into the relationship between enhancer elements. Our method opens the door for studying multi-loci interactions and their impact on gene regulation in other biological settings.

Original languageEnglish (US)
Pages (from-to)8714-8725
Number of pages12
JournalNucleic Acids Research
Volume44
Issue number18
DOIs
StatePublished - Oct 14 2016

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Antigen Receptors
DNA
Cell Communication
Genes
Chromatin
Chromosomes
Population
Datasets

ASJC Scopus subject areas

  • Genetics

Cite this

Jiang, T., Raviram, R., Snetkova, V., Rocha, P. P., Proudhon, C., Badri, S., ... Kluger, Y. (2016). Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions. Nucleic Acids Research, 44(18), 8714-8725. https://doi.org/10.1093/nar/gkw568

Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions. / Jiang, Tingting; Raviram, Ramya; Snetkova, Valentina; Rocha, Pedro P.; Proudhon, Charlotte; Badri, Sana; Bonneau, Richard; Skok, Jane A.; Kluger, Yuval.

In: Nucleic Acids Research, Vol. 44, No. 18, 14.10.2016, p. 8714-8725.

Research output: Contribution to journalArticle

Jiang, T, Raviram, R, Snetkova, V, Rocha, PP, Proudhon, C, Badri, S, Bonneau, R, Skok, JA & Kluger, Y 2016, 'Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions', Nucleic Acids Research, vol. 44, no. 18, pp. 8714-8725. https://doi.org/10.1093/nar/gkw568
Jiang, Tingting ; Raviram, Ramya ; Snetkova, Valentina ; Rocha, Pedro P. ; Proudhon, Charlotte ; Badri, Sana ; Bonneau, Richard ; Skok, Jane A. ; Kluger, Yuval. / Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions. In: Nucleic Acids Research. 2016 ; Vol. 44, No. 18. pp. 8714-8725.
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