Identification of a Streptococcus sanguis receptor for salivary agglutinins.

M. R. Robinovitch, Daniel Malamud, B. Rosan, E. E. Golub, P. Lancy

Research output: Contribution to journalArticle

Abstract

The objective of this study was to characterize a fraction from oral streptococci containing receptor activity for salivary agglutinin molecules. Several species and strains of streptococci were disrupted in a Ribi press. The supernatant was nuclease-treated and subjected to differential centrifugation. Receptor activity in the fractions was measured by the inhibition of saliva-mediated bacterial aggregation. In addition, bacterial strains were tested for their ability to aggregate and to deplete saliva of agglutinin activity. Three patterns of activity were observed: Streptococcus sanguis M5 depleted saliva of agglutinin activity and aggregated well; Streptococcus sanguis CC5A depleted saliva of agglutinin but did not aggregate well; and Streptococcus faecalis S-161 neither depleted saliva of agglutinin nor did it aggregate. The 105,000 g supernatant fractions derived from Ribi-disrupted Streptococcus sanguis M5 and CC5A, but not from Streptococcus faecalis, showed dose-dependent inhibition of saliva-mediated aggregation. This inhibitory activity was non-dialyzable, had the same heat and trypsin sensitivity as that seen with intact bacteria, and was not due to enzymatic digestion of the salivary agglutinin. Iso-electric focusing revealed a single active region with a pI of 5.5 which was clearly separated from the bulk of the bacterial proteins.

Original languageEnglish (US)
Pages (from-to)98-104
Number of pages7
JournalJournal of Dental Research
Volume65
Issue number2
StatePublished - Feb 1986

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Streptococcus sanguis
Agglutinins
Saliva
Enterococcus faecalis
Streptococcus
Bacterial Proteins
Centrifugation
Trypsin
Digestion
Hot Temperature
Bacteria

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Robinovitch, M. R., Malamud, D., Rosan, B., Golub, E. E., & Lancy, P. (1986). Identification of a Streptococcus sanguis receptor for salivary agglutinins. Journal of Dental Research, 65(2), 98-104.

Identification of a Streptococcus sanguis receptor for salivary agglutinins. / Robinovitch, M. R.; Malamud, Daniel; Rosan, B.; Golub, E. E.; Lancy, P.

In: Journal of Dental Research, Vol. 65, No. 2, 02.1986, p. 98-104.

Research output: Contribution to journalArticle

Robinovitch, MR, Malamud, D, Rosan, B, Golub, EE & Lancy, P 1986, 'Identification of a Streptococcus sanguis receptor for salivary agglutinins.', Journal of Dental Research, vol. 65, no. 2, pp. 98-104.
Robinovitch, M. R. ; Malamud, Daniel ; Rosan, B. ; Golub, E. E. ; Lancy, P. / Identification of a Streptococcus sanguis receptor for salivary agglutinins. In: Journal of Dental Research. 1986 ; Vol. 65, No. 2. pp. 98-104.
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