Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis

Bo Li, Caitlin Byrt, Jiaen Qiu, Ute Baumann, Maria Hrmova, Aurelie Evrard, Alexander A T Johnson, Kenneth Birnbaum, Gwenda M. Mayo, Deepa Jha, Sam W. Henderson, Mark Tester, Mathew Gilliham, Stuart J. Roy

Research output: Contribution to journalArticle

Abstract

Under saline conditions, higher plants restrict the accumulation of chloride ions (Cl) in the shoot by regulating their transfer from the root symplast into the xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen of salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified a candidate gene involved in Clxylem loading from the Nitrate transporter 1/Peptide Transporter family (NPF2.4). This gene was highly expressed in the root stele compared to the cortex, and its expression decreased after exposure to NaCl or abscisic acid. NPF2.4 fused to fluorescent proteins, expressed either transiently or stably, was targeted to the plasma membrane. Electrophysiological analysis of NPF2.4 inXenopus laevisoocytes suggested that NPF2.4 catalyzed passive Clefflux out of cells and was much less permeable to NO3 . Shoot Claccumulation was decreased followingNPF2.4artificial microRNA knockdown, whereas it was increased by overexpression ofNPF2.4. Taken together, these results suggest that NPF2.4 is involved in long-distance transport of Clin plants, playing a role in the loading and the regulation of Clloading into the xylem of Arabidopsis roots during salinity stress.

Original languageEnglish (US)
Pages (from-to)1014-1029
Number of pages16
JournalPlant Physiology
Volume170
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

transport proteins
Arabidopsis
Chlorides
Xylem
Carrier Proteins
chlorides
shoots
xylem
Abscisic Acid
peptide transporters
Salinity
MicroRNAs
symplast
stele
Genes
apoplast
Salts
microRNA
Cell Membrane
sodium chloride

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis. / Li, Bo; Byrt, Caitlin; Qiu, Jiaen; Baumann, Ute; Hrmova, Maria; Evrard, Aurelie; Johnson, Alexander A T; Birnbaum, Kenneth; Mayo, Gwenda M.; Jha, Deepa; Henderson, Sam W.; Tester, Mark; Gilliham, Mathew; Roy, Stuart J.

In: Plant Physiology, Vol. 170, No. 2, 01.02.2016, p. 1014-1029.

Research output: Contribution to journalArticle

Li, B, Byrt, C, Qiu, J, Baumann, U, Hrmova, M, Evrard, A, Johnson, AAT, Birnbaum, K, Mayo, GM, Jha, D, Henderson, SW, Tester, M, Gilliham, M & Roy, SJ 2016, 'Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis', Plant Physiology, vol. 170, no. 2, pp. 1014-1029. https://doi.org/10.1104/pp.15.01163
Li, Bo ; Byrt, Caitlin ; Qiu, Jiaen ; Baumann, Ute ; Hrmova, Maria ; Evrard, Aurelie ; Johnson, Alexander A T ; Birnbaum, Kenneth ; Mayo, Gwenda M. ; Jha, Deepa ; Henderson, Sam W. ; Tester, Mark ; Gilliham, Mathew ; Roy, Stuart J. / Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis. In: Plant Physiology. 2016 ; Vol. 170, No. 2. pp. 1014-1029.
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