Identification of a novel amidase motif in neutral ceramidase

Sehamuddin Galadari, Bill X. Wu, Cungui Mao, Patrick Roddy, Samer El Bawab, Yusuf A. Hannun

Research output: Contribution to journalArticle

Abstract

Neutral CDases (ceramidases) are newly identified enzymes with important roles in cell regulation, but little is known about their catalytic mechanisms. In the present study the full-length human neutral CDase was cloned and expressed in the yeast double-knockout strain Δypc1Δydc1, which lacks the yeast CDases YPC1p and YDC1p. Biochemical characterization of the human neutral CDase showed that the enzyme exhibited classical Michaelis-Menten kinetics, with an optimum activity at pH 7.5. Activity was enhanced by Na + and Ca2+. Mg2+ and Mn2+ were somewhat stimulatory, but Zn2+, Cu2+ and Fe2+ inhibited the enzyme. Dithiothreitol and 2-mercaptoethanol dose-dependently inhibited neutral CDase. In order to identify which amino acids were involved in the catalytic action of neutral CDase, the purified enzyme was subjected to chemical modifications. It was observed that the serine residue modifier di-isopropyl fluorophosphate dose-dependently inhibited activity, implicating a serine residue in the catalytic action. From an alignment of the sequences of the neutral CDases from different species, all conserved serine residues were selected for site-directed mutagenesis. Of the six aligned serine residues that were mutated to alanine, only the S354A mutant lost its activity totally. Ser354 falls within a very highly conserved hexapeptide sequence GDVSPN, which itself was in the middle of a larger conserved sequence, namely NXGDVSPNXXGP/XXC. Moreover, mutations of Asp352 and Cys362 in the consensus sequence to alanine resulted in loss of activity of neutral CDase. Hence the present study identified a novel amidase sequence containing a critical serine residue that may function as a nucleophile in the hydrolytic attack on the amide bond present in ceramide.

Original languageEnglish (US)
Pages (from-to)687-695
Number of pages9
JournalBiochemical Journal
Volume393
Issue number3
DOIs
StatePublished - Feb 1 2006

Fingerprint

amidase
Neutral Ceramidase
Serine
Conserved Sequence
fluorophosphate
Enzymes
Alanine
Yeast
Ceramidases
Yeasts
Mutagenesis
Nucleophiles
Mercaptoethanol
Sequence Alignment
Ceramides
Dithiothreitol
Consensus Sequence
Chemical modification
Site-Directed Mutagenesis
Amides

Keywords

  • Catalytically important serine residue
  • DihydroCDase (YDC1p)
  • Neutral ceramidase
  • Novel amidase motif
  • Phytocdase (YPC1p)
  • Sphingosine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Galadari, S., Wu, B. X., Mao, C., Roddy, P., El Bawab, S., & Hannun, Y. A. (2006). Identification of a novel amidase motif in neutral ceramidase. Biochemical Journal, 393(3), 687-695. https://doi.org/10.1042/BJ20050682

Identification of a novel amidase motif in neutral ceramidase. / Galadari, Sehamuddin; Wu, Bill X.; Mao, Cungui; Roddy, Patrick; El Bawab, Samer; Hannun, Yusuf A.

In: Biochemical Journal, Vol. 393, No. 3, 01.02.2006, p. 687-695.

Research output: Contribution to journalArticle

Galadari, S, Wu, BX, Mao, C, Roddy, P, El Bawab, S & Hannun, YA 2006, 'Identification of a novel amidase motif in neutral ceramidase', Biochemical Journal, vol. 393, no. 3, pp. 687-695. https://doi.org/10.1042/BJ20050682
Galadari, Sehamuddin ; Wu, Bill X. ; Mao, Cungui ; Roddy, Patrick ; El Bawab, Samer ; Hannun, Yusuf A. / Identification of a novel amidase motif in neutral ceramidase. In: Biochemical Journal. 2006 ; Vol. 393, No. 3. pp. 687-695.
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