Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate

Katerina Ragkousi, Patrick Eichenberger, Christiaan Van Ooij, Peter Setlow

Research output: Contribution to journalArticle

Abstract

Bacillus subtilis spores can germinate with a 1:1 chelate of Ca2+ and dipicolinic acid (DPA), a compound present at high levels in the spore core. Using a genetic screen to identify genes encoding proteins that are specifically involved in spore germination by Ca2+-DPA, three mutations were identified. One was in the gene encoding the cortex lytic enzyme, CwlJ, that was previously shown to be essential for spore germination by Ca2+-DPA. The other two were mapped to an open reading frame, ywdL, encoding a protein of unknown function. Analysis of ywdL expression showed that the gene is expressed during sporulation in the mother cell compartment of the sporulating cell and that its transcription is σE dependent. Functional characterization of YwdL demonstrated that it is a new spore coat protein that is essential for the presence of CwlJ in the spore coat. Assembly of YwdL itself into the spore coat is dependent on the coat morphogenetic proteins CotE and SpoIVA. However, other than lacking CwlJ, ywdL spores have no obvious defect in their spore coat. Because of the role for YwdL in a part of the spore germination process, we propose renaming ywdL as a spore germination gene, gerQ.

Original languageEnglish (US)
Pages (from-to)2315-2329
Number of pages15
JournalJournal of Bacteriology
Volume185
Issue number7
DOIs
StatePublished - Apr 2003

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Essential Genes
Germination
Bacillus subtilis
Spores
Capsid Proteins
Open Reading Frames
Genes
Proteins
Stem Cells
Gene Expression

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate. / Ragkousi, Katerina; Eichenberger, Patrick; Van Ooij, Christiaan; Setlow, Peter.

In: Journal of Bacteriology, Vol. 185, No. 7, 04.2003, p. 2315-2329.

Research output: Contribution to journalArticle

Ragkousi, Katerina ; Eichenberger, Patrick ; Van Ooij, Christiaan ; Setlow, Peter. / Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 7. pp. 2315-2329.
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